Cellooligosaccharide Synthesis Using Sucrose and Phosphate Control
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Solution Overview
Problem
Existing methods for producing cellooligosaccharides using cellodextrin phosphorylase are costly due to the high expense of α-glucose-1-phosphate and result in low yield.
Innovation Solution
A two-stage enzymatic process using sucrose phosphorylase and cellodextrin phosphorylase, where sucrose generates α-glucose-1-phosphate, and then α-glucose-1-phosphate is polymerized into cellooligosaccharides with controlled phosphoric acid concentration to enhance yield.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of manufacture
If α-glucose-1-phosphate is used as a starting material for cellooligosaccharide synthesis, then the synthesis can proceed using cellodextrin phosphorylase, but the production cost becomes very high and the yield is not necessarily high
Solution Approach 1:
The patent changes the starting material from expensive α-glucose-1-phosphate to inexpensive sucrose, and optimizes the phosphoric acid concentration parameter (3-120 mol/m³) to achieve both cost reduction and high yield. This parameter change transforms the economic feasibility of the synthesis process.
Solution Approach 2:
The patent introduces phosphoric acid as an intermediary substance that enables the conversion of sucrose to α-glucose-1-phosphate in situ, which then serves as the actual substrate for cellooligosaccharide synthesis. This intermediary approach allows using cheap sucrose instead of expensive α-glucose-1-phosphate directly.
2Productivity
If α-glucose-1-phosphate is used as a starting material, then the synthesis reaction can proceed, but the percentage yield of cellooligosaccharide is not necessarily high
Solution Approach 1:
The patent optimizes the phosphoric acid concentration to a specific range (3-120 mol/m³) to maximize the percentage yield of cellooligosaccharide. This parameter optimization ensures that the synthesis reaction proceeds efficiently with high yield, resolving the contradiction between reaction progress and product yield.
3Speed
If phosphoric acid concentration is increased to promote the reaction, then the reaction rate may improve, but phosphorolysis increases which reduces the percentage yield
Solution Approach 1:
The patent identifies and optimizes the phosphoric acid concentration parameter to a specific range (3-120 mol/m³) that balances the reaction rate and phosphorolysis. This optimized parameter range ensures sufficient reaction speed while minimizing the harmful phosphorolysis effect, thereby achieving high percentage yield.
Solution Approach 2:
The patent establishes a feedback mechanism where the phosphoric acid concentration is controlled within a specific range based on its dual role: promoting the synthesis reaction while preventing excessive phosphorolysis. This feedback control of the concentration parameter resolves the contradiction between reaction rate and yield.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method produces cellooligosaccharides inexpensively with high yield by utilizing inexpensive sucrose and controlling phosphoric acid concentration to suppress phosphorolysis, thereby increasing the percentage yield.
Implementation Method 1
sucrose phosphorylase is used to generate, from sucrose, α-glucose-1-phosphate
Implementation Method 2
cellodextrin phosphorylase is used to generate a cellooligosaccharide, from the obtained α-glucose-1-phosphate and the primer
Data Source
AI summary
A method by which a cellooligosaccharide can be produced inexpensively at high percentage yield is provided. In the method for producing a cellooligosaccharide according to an embodiment, at least one primer selected from the group consisting of glucose, cellobiose, and derivatives in which anomeric positions of glucose and cellobiose are modified and sucrose are subjected to, in the presence of phosphoric acid, actions of sucrose phosphorylase and cellodextrin phosphorylase. In this case, in the reaction system, the concentration of phosphoric acid is set to 3 mol/m3 or more and 120 mol/m3 or less.