Fermentation TRIZ Cases

A fused endoglucanase and cellulose-binding domain improves denim biostoning at lower dosage while reducing indigo backstaining and fabric damage.

Band gap grading through a subcell active layer improves current collection, voltage, and radiation resistance in space multijunction solar cells.

Band gap grading through a subcell active layer improves current collection, fill factor, and radiation resistance in space solar cells.

A graded band gap across a subcell active layer boosts short circuit current, fill factor, and radiation hardness in space solar cells.

A graded band gap from surface to junction boosts current collection and helps multijunction solar cells retain power output after radiation exposure.

Engineered host cells use an enzyme cascade to convert succinate and acetate into adipate intermediates with lower toxicity and side-product loss.

Acid pre-treatment removes AAEM minerals from date pits before pyrolysis, enabling high levoglucosan yield without added catalysts.

Ligand-bound aptamers block RNase P cleavage to switch target RNA expression on, enabling precise and safer gene regulation.

Dual enzymatic hydrolysis with Savinase 16L and alkaline protease raises tilapia skull chondroitin sulfate yield while limiting degradation and pollution.

RNA trans-splicing delivered by rAAV replaces defective exons in large ocular genes, enabling mutation correction beyond standard gene therapy limits.

Through-washing a compressed PHA filter cake to pH 4.0-5.5 removes catalyst impurities and preserves thermal stability without corrosion-resistant equipment.

Weakening ArgR-related protein activity in Corynebacterium lifts repression of L-citrulline biosynthesis and improves fermentation yield.

Engineered microbial cells synthesize sialic acid intracellularly to produce sialylated saccharides without exogenous feedstocks or UDP-GlcNAc.

Using sucrose to generate α-glucose-1-phosphate in situ, this case raises cellooligosaccharide yield while lowering raw material cost.

A two-compartment bipolar electrodialysis layout converts monovalent organic acid salts with less fouling, lower water use, and high purity.

A light-gated yeast evolution platform selects TEV protease variants with faster cleavage while preserving sequence specificity for FLARE and SPARK.

Controlling reducing sugar at 5 g/L or less during cell culture cuts protein glycation and improves recombinant polypeptide homogeneity.

Using glucose-1-phosphate and beta-glucan phosphorylase, this case shows reproducible beta-1,2-oligoglucan production with controlled viscosity.

Using HaHex74 and β-galactosidase, this case shows food-grade LNT2 and LNnT synthesis from chitin hydrolysate and lactose with higher yield.

Engineered recombinant cells express terpenoid synthase genes to produce high-purity nerolidol with far higher fermentation yield.

Immobilized fructosyltransferase converts sucrose in juice into fructooligosaccharides, cutting sugar while preserving flavor and nutrition.

Continuous sucrose feeding and membrane fructose removal help control alternan-oligosaccharide chain length while limiting by-product buildup.

Hybrid fermentation and in-medium transglycosidase recycling produce complex HMOs with lower lactose buildup, simpler purification, and higher yield.

Enzymatic sucrose-to-amylose conversion bypasses starch feedstocks to raise gamma-cyclodextrin yield, reduce byproducts, and simplify purification.

Heterologous mitochondria or chloroplasts in cell-free extracts maintain cofactor balance, extending reaction time and boosting expression yield.

Specific PglB amino acid substitutions boost saccharide transfer to glycosylation motifs, raising N-glycosylated protein yield.

Alternating cinnamic acid and ricinoleic acid units in a biomass-derived polyester resolves the heat resistance versus flexibility tradeoff.

Modified cupin nitroaldolases sustain >99% enantiomeric excess in the Henry reaction while reaching up to 97.3% yield.

Polymerizing low-purity organic acid first, then washing out inorganic compounds, enables high-purity prepolymers and polymers without costly purification.

Adjusting pH and conductivity enables selective chromatography binding, raising batroxobin purity, activity, and yield while shortening purification time.

A cell-specific inducible caspase-9 system rapidly removes target cells without harming non-target human cells, enabling replacement organ production.

An oxidoreductase route uses xH+ from D2O or T2O to make biologically active labeled NAD(P)H selectively, without hazardous reductants.

Chaotropic precipitation and filtration in one well remove transfer steps, cutting contamination risk, waste, and polynucleotide loss.

Heat treatment above 105°C lowers sulfur dioxide in konjac-derived mannose or mannooligosaccharide compositions to meet food distribution limits.

A three-compartment bipolar electrodialysis stack converts monovalent acid salts with less fouling, lower water use, and high acid purity.

Nuclease-resistant end nucleotides protect long ssDNA cassettes from exonuclease degradation while enabling assembly of stable in vivo delivery constructs.

Low-salt flow-through HIC removes residual protein before oligo-dT capture, improving mRNA purity and chromatography productivity.

Heat treatment at 105°C or higher lowers sulfur dioxide in konjac-derived mannose and mannooligosaccharide compositions to food-safe levels.

Bacterial β-galactoside α-sialyltransferases avoid CMP-Neu5Ac hydrolysis, improving sialylated glycan yield and reducing by-products.

Artificially fused FabA-FabZ dimers improve enzyme solubility and cooperativity in E. coli, enabling higher fatty acid biosynthesis.

Inert-gas slurry processing and refractance window drying preserve cannabinoids while stabilizing wet-harvested hemp and cannabis.

Co-expressing BiP, Grp170, and ERdj3-like helper factors in yeast boosts heterologous protein folding, secretion, and yield.

Endo-polygalacturonase plus microfiltration and nanofiltration turns potato pulp into purified HG- and RG-I-rich saccharide fractions.

Engineered GT and SuS enzymes reduce glycosyl donor demand and avoid complex nucleotide recycling in scalable rebaudioside synthesis.

Sequential bacteria build a polysaccharide network and mineralize calcium carbonate to form tunable bone-mimetic composites for tissue engineering.

Anion exchange separation by sialic acid count avoids difficult glycan synthesis and yields purified complex-type glycoasparagine.

Modular enzymatic N-glycan assembly replaces costly purification and chemical synthesis to produce homogeneous glycoproteins at milligram scale.

Impeller torque guides when to add surfactant, pH adjuster, and enzyme, improving cellulose wetting without hindering saccharification.

Moderate centrifugal separation retains native membrane vesicles in cell-free extracts, boosting glycoprotein yield and simplifying processing.

Low-temperature CO2 and H2 fermentation boosts short-chain fatty acid yield, suppresses methane, and lowers PHA feedstock cost.

Binding mRNA to a stationary phase couples capping, washing, and elution to cut loss, simplify processing, and improve capped mRNA stability.

Highly concentrated aqueous salt suppresses racemic background reactions, enabling high enantiomeric excess for optically active cyanohydrins.

Novel switchgrass promoters enable strong constitutive gene expression across monocots, dicots, and ferns without species-specific development.

Replacing halogenated solvents with dimethyl carbonate reduces environmental harm while maintaining high-purity PHA recovery yields.

Continuous extraction removes caprylic acid from bioreactor broth to shift microbiome metabolism toward higher chain elongation.

Specific nucleotide sequences improve protein expression by overcoming transcription inhibition from the NPT303 5'-UTR.