Stabilizing Cellulose Synthase Gene in Acetic Acid Bacteria
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Solution Overview
Problem
Wild-type acetic acid bacteria often mutate into Cel− strains that lose the ability to produce cellulose during shaking culture, reducing the efficiency of cellulose production.
Innovation Solution
A recombinant microorganism with a genetic modification that inactivates the insertion sequence recognition site in the cellulose synthase A gene, stabilizing cellulose production by reducing the occurrence of cellulose non-producing mutant strains.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If wild-type acetic acid bacteria are used for cellulose production, then cellulose can be produced initially, but the bacteria mutate into Cel- strains during shaking culture, losing the ability to produce cellulose
Solution Approach 1:
The patent applies preliminary action by inactivating the IS recognition site in the cellulose synthase A gene before cellulose production begins. This preventive genetic modification ensures that insertion sequences cannot insert into the gene during culture, thereby preventing mutation to Cel- strains and maintaining stable cellulose production throughout the cultivation process
Solution Approach 2:
The patent extracts or removes the functional IS recognition site from the cellulose synthase A gene through genetic modification. By eliminating this recognition site, the patent prevents the harmful insertion of transposons that would otherwise cause gene inactivation and loss of cellulose production capability
2Reliability
If the insertion sequence recognition site in cellulose synthase A gene is inactivated through genetic modification, then the occurrence of cellulose non-producing mutant strains is reduced, but the complexity of strain preparation increases
Solution Approach 1:
The patent applies parameter changes by modifying the nucleotide sequence of the IS recognition site in the cellulose synthase A gene. Specific nucleotides are altered to prevent recognition by transposase enzymes, thereby blocking insertion sequence integration. This sequence parameter modification creates a stable genetic configuration that prevents mutant strain formation while maintaining a relatively simple preparation process
Data Source
AI summary
Provided is a recombinant microorganism having enhanced cellulose synthase gene stability, a method of producing cellulose by using the recombinant microorganism, and a method of preparing the recombinant microorganism.