Simultaneous Coagulation Protease Detection via Spectral Separation
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Solution Overview
Problem
Current coagulation diagnostic methods require separate tests to determine the activity or inhibition of thrombin and factor Xa, which is time-consuming and prone to variations, necessitating a method for simultaneous determination in a single test reaction.
Innovation Solution
A method involving a sample mixed with specific chromogenic substrates for thrombin and factor Xa, where the chromophores have absorption maxima differing by at least 100 nm, allowing for spectral separation and photometric determination at different wavelengths, enabling simultaneous measurement of both coagulation factors in a single test.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If separate tests are performed to determine thrombin and factor Xa activity, then measurement precision is maintained, but loss of time and productivity decrease
Solution Approach 1:
The patent combines separate tests for thrombin and factor Xa into a single simultaneous determination test. Multiple chromogenic substrates with different absorption maxima are used in the same reaction mixture, allowing both coagulation factors to be measured at the same time through spectrophotometric detection at different wavelengths, thereby reducing time loss while maintaining measurement precision
Solution Approach 2:
The patent introduces a new dimension of spectral wavelength separation to enable simultaneous measurement. By selecting chromogenic substrates with absorption maxima differing by at least 100 nm, the test distinguishes between thrombin and factor Xa activities along the wavelength dimension, allowing parallel determination without interference
2Measurement precision
If separate tests are performed for thrombin and factor Xa, then measurement reliability is maintained, but productivity decreases
Solution Approach 1:
The patent merges multiple coagulation factor determinations into a single test reaction. By using chromogenic substrates specific for different coagulation factors (thrombin, factor Xa) with spectrally separable chromophores, the system achieves simultaneous measurement that improves productivity while maintaining reliability through controlled reaction conditions and wavelength-specific detection
Solution Approach 2:
The patent creates a universal test system that can determine multiple coagulation factors simultaneously. The reaction mixture serves multiple functions: it contains substrates for different factors, buffers the reaction, and enables detection at multiple wavelengths, making the single test applicable for comprehensive coagulation profiling
3Measurement precision
If chromogenic substrates with different absorption maxima are used, then spectral separation is achieved, but device complexity increases
Solution Approach 1:
The patent changes the spectral parameter of the chromogenic substrates by selecting substrates with absorption maxima differing by at least 100 nm. This parameter change enables clear spectral separation for simultaneous detection of multiple coagulation factors. The complexity is managed by using standard spectrophotometric detection at fixed wavelengths rather than requiring complex spectral analysis equipment
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach reduces material consumption and time, while minimizing errors by allowing for the simultaneous determination of thrombin and factor Xa activity or inhibition under the same conditions, providing accurate and reliable results.
Implementation Method 1
the first chromomeric substrate has a chromospheres whose absorption maximum differs by at least 100 nm from the absorption maximum of the chromospheres of the second chromomeric substrate... allowing for spectral separation and photometric determination at different wavelengths
Data Source
AI summary
The present invention relates to a chromogenic method for simultaneously determining the activity of multiple coagulation proteases or for simultaneously determining the inhibition of multiple coagulation proteases in a single test reaction. For this purpose, use is made of two chromogenic substrates which have different absorption maxima and whose color signals can be separated spectrally.