Colloid Continuous Phase Molecule Concentration Measurement
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Solution Overview
Problem
Current methods for evaluating the interaction between particles and molecules in colloids are limited by restricted experimental conditions, excluding the study of low molecular mass interactions and non-metallic particulate systems, and require improved techniques for measuring molecule concentrations in the continuous phase.
Innovation Solution
A process and kit for obtaining a monophasic liquid composition equivalent to the continuous phase of a colloid, allowing for the measurement of molecule concentrations using test and control samples, which enables accurate interaction studies between molecules and particles by minimizing residual compounds and using particle-free solutions.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If analytical ultracentrifugation techniques are used to evaluate interactions, then changes in sedimentation coefficient can be determined, but the study of interactions between particles and low molecular mass molecules is excluded
Solution Approach 1:
The patent develops a methodology that can evaluate interactions between colloidal particles and molecules of any molecular mass (including low molecular mass molecules) by measuring concentration changes in the continuous phase. This universal approach replaces the mass-specific limitation of analytical ultracentrifugation, allowing the same technique to study proteins, peptides, small molecules, and other colloidal systems uniformly.
2Measurement precision
If LSPs resonance spectrometry is used to evaluate interactions, then changes in wavelength of maximum absorbance can be detected, but applications are limited to metal particles with LSP
Solution Approach 1:
The patent creates a universal measurement methodology that works with any colloidal system (metallic, non-metallic, organic, inorganic) by measuring concentration changes in the continuous phase rather than relying on particle-specific optical properties like LSP resonance. This allows dendrimers, liposomes, protein dispersions, and other non-metallic colloids to be studied with the same approach used for metal nanoparticles.
Solution Approach 2:
The patent replaces the optical detection mechanism (LSP resonance wavelength shifts) with a concentration-based measurement approach in the continuous phase. By measuring how molecule concentration changes in the continuous phase during interaction, the method substitutes particle-specific optical effects with a universal concentration measurement that works for all colloidal systems.
3Measurement precision
If conventional methods are used to measure molecule concentrations in colloids, then interaction evaluation is limited, but accurate concentration measurement in the continuous phase is achieved
Solution Approach 1:
The patent extracts the measurement focus from the colloidal particles themselves to the continuous phase surrounding them. By measuring concentration changes of molecules in the continuous phase (rather than trying to detect particle-molecule complexes directly), the method accurately quantifies interactions while maintaining applicability to diverse colloidal systems and molecular masses.
Data Source
AI summary
A method for measuring a concentration of molecules, characterized in that the method measures the concentration of molecules dissolved in a continuous phase of a colloid and includes obtaining a test sample by mixing a number of molecules with a volume of colloid, obtaining a control sample by mixing a number of molecules with a volume of a composition comprising a particle-free solution extracted from a fraction of the continuous phase of same colloid used in the obtaining the test sample, so that a value of the concentration of molecules in the mixture is equal to the value of the concentration of molecules in the test sample obtained in the obtaining the test sample, and submitting the test and the control samples obtained in the obtaining the test sample and obtaining the control sample to a process in order to concentrate the particles of the test sample.