Methods relating to tissue regeneration
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Solution Overview
Problem
Existing methods for bladder augmentation using cell-seeded scaffolds face challenges in acquiring viable cells, leading to issues like stone formation, infections, and malignant transformation, while cell-free total conditioned media (TCM) derived from co-cultured mesenchymal stem cells (MSC) and CD34+ hematopoietic stem/progenitor cells (HSPC) are needed to promote bladder tissue regeneration.
Innovation Solution
Administering MSC/CD34+ HSPC co-cultured TCM to subjects, which includes substances like cytokines, chemokines, and extracellular matrix components, to treat and prevent bladder damage, trauma, or loss, and optionally providing a graft, with administration methods such as instillation and timed release from scaffolds.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If cell-seeded scaffolds are used for bladder augmentation, then tissue regeneration is promoted, but complications such as stone formation, infections, and malignant transformation occur
Solution Approach 1:
The patent extracts and isolates the beneficial paracrine factors and signaling molecules from the conditioned media of co-cultured MSCs and HSPCs, separating these regenerative components from the actual cells themselves. This allows the therapeutic effects to be delivered without the harmful complications associated with cell-based therapies, effectively taking out the useful properties while leaving behind the problematic cellular components.
Solution Approach 2:
The conditioned media serves as an intermediary carrier that transfers the regenerative signals from the co-cultured stem cells to the damaged bladder tissue. Instead of directly implanting cells that may cause complications, the patent uses the media as a mediator to deliver the same regenerative benefits through paracrine factors, growth factors, and cytokines without the risks of cell-based therapy.
2Reliability
If viable cells are acquired for scaffold seeding, then tissue regeneration is enhanced, but acquisition challenges and procedural complexity increase
Solution Approach 1:
Instead of using actual living cells that require complex acquisition, culture, and implantation procedures, the patent creates a copy of the cellular function through conditioned media. The media captures and preserves the regenerative signaling capacity of the co-cultured cells, providing a simplified alternative that replicates the therapeutic effect without the procedural complexity of cell handling and scaffold seeding.
3Reliability
If traditional cell-based therapy is used, then regenerative effect is achieved, but long-term safety and stability are compromised
Solution Approach 1:
The patent converts the harmful potential of living cells (which can cause malignancy, infection, and stone formation) into a beneficial cell-free therapy. By utilizing the conditioned media that cells naturally produce, the invention captures the regenerative benefits while eliminating the safety risks, effectively turning a potential harm into a therapeutic advantage.
Data Source
AI summary
The present disclosure provides methods related to tissue regeneration using human mesenchymal stem cell (MSC)/CD34+ hematopoietic stem/progenitor cell (HSPC) co-cultured total conditioned media. In particular, the present disclosure provides novel methods for treating and/or preventing damage to, trauma to, and/or loss of tissue.