Continuous Protein Hydrolysis for Low-Viscosity Sterile Nutrition

Resolve Bottlenecks,
Find Innovative Solutions
Generate Solutions

Solution Overview

Problem

Existing protein hydrolysis processes result in compositions with unpredictable and often high viscosity and bitterness due to varying degrees of hydrolysis, making them difficult to control and consume, especially in high protein content nutritional products.

Innovation Solution

A continuous process using endopeptidase for a finite time followed by heat inactivation, achieving a narrow range of protein hydrolysis and low viscosity, resulting in a heat-sterilized composition with 20-50% protein hydrolysis and reduced bitterness.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Quantity of substance

If the concentration of proteins in a nutritional liquid composition is increased, then the protein content is improved, but the overall product viscosity increases

Engineering Contradiction:
Improveprotein contentVSAvoidviscosity
Core Design Contradiction:
Quantity of substanceVSStability of the object's composition

Solution Approach 1:

The patent applies segmentation by hydrolyzing the protein into smaller peptide fragments and amino acids through enzymatic treatment. This breaks down the large protein molecules into smaller units that do not aggregate as much, thereby maintaining lower viscosity even at high protein concentrations (5-20% by weight). The continuous hydrolysis process ensures controlled fragmentation throughout the production line.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent utilizes parameter changes by controlling the degree of protein hydrolysis through continuous enzymatic treatment with specific parameters (temperature, time, enzyme concentration). By optimizing these parameters, the protein is hydrolyzed to a extent that reduces molecular size and intermolecular interactions, thereby controlling viscosity while preserving nutritional value and stability during shelf life.

Inventive Principle:
Principle #35Parameter changes

2Quantity of substance

If the concentration of proteins in a nutritional liquid composition is increased, then the protein content is improved, but the stability deteriorates due to salt crystal formation

Engineering Contradiction:
Improveprotein contentVSAvoidstability
Core Design Contradiction:
Quantity of substanceVSStability of the object's composition

Solution Approach 1:

The continuous hydrolysis process segments proteins into smaller peptides and amino acids that remain in solution rather than forming salt crystals during storage. This molecular fragmentation prevents the crystallization phenomenon that would otherwise occur with intact proteins at high concentrations, thereby maintaining product stability for at least 9 months shelf life.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent employs continuous hydrolysis throughout the production process, ensuring that protein hydrolysis is completed before packaging. This continuous action ensures complete hydrolysis of all proteins in the batch, preventing subsequent crystal formation during storage and maintaining long-term stability without requiring additional stabilization steps.

Inventive Principle:
Principle #20Continuity of useful action

3Manufacturing precision

If conventional batch protein hydrolysis processes are used, then the protein source is hydrolyzed, but the degree of hydrolysis is unpredictable and the product is bitter in taste

Engineering Contradiction:
Improvedegree of hydrolysis controlVSAvoidbitterness
Core Design Contradiction:
Manufacturing precisionVSObject-generated harmful factors

Solution Approach 1:

The patent transitions from batch to continuous hydrolysis, maintaining constant enzymatic action throughout the process. This continuous treatment ensures uniform and predictable degree of hydrolysis (20-50% as measured by NPN/TN ratio) across all batches, eliminating the variability inherent in batch processes. The consistent hydrolysis level prevents over-hydrolysis that would generate excessive bitter-tasting peptides.

Inventive Principle:
Principle #20Continuity of useful action

Solution Approach 2:

The continuous hydrolysis process incorporates monitoring and control mechanisms that measure the degree of hydrolysis in real-time and adjust enzyme activity accordingly. This feedback control ensures the hydrolysis remains within the optimal range (20-50% NPN/TN), preventing progression to levels that会产生 excessive bitterness while maintaining manufacturing precision and product quality consistency.

Inventive Principle:
Principle #23Feedback

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The process provides a reliable and controllable degree of hydrolysis, producing a low viscosity, heat-sterilized composition suitable for nutritional products with improved palatability and stability, suitable for enteral and parenteral use.

Implementation Method 1

Performing a continuous hydrolysis step wherein the aqueous solution of a protein source is treated with at least one endopeptidase

Methodology Applied
Scientific EffectHydrolysis: Hydrolysis

Implementation Method 2

heat inactivation of the endopeptidase

Methodology Applied
Scientific EffectHeat inactivation: Heat Treatment

Data Source

PatentEP3389392B1Heat sterilized high protein compositions with hydrolyzed protein from a continuous process with at least one endopeptidase
Publication Date: 2026.03.25 SOCIETE DES PRODUITS NESTLE SA
  • EP3389392B1 patent drawingFigure 1
  • EP3389392B1 patent drawingFigure 2~3

AI summary

The present invention is directed to a heat sterilized composition comprising: a protein source in an amount of 5 to 20% by weight of the composition, said protein source comprising hydrolyzed protein; wherein the protein source has been treated in a continuous process with at least one endopeptidase, typically for a finite length of time. Furthermore, the invention concerns a heat sterilized high protein composition having low viscosity and being devoid of bitterness, comprising hydrolyzed heat sensitive protein source having a degree of protein hydrolysis in NPN/TN of 20 to 50% and at least one heat inactivated endopeptidase. The present invention is furthermore directed to a continuous process for preparing a heat sterilized composition which comprises a protein source containing hydrolyzed protein, the process comprising the following steps: (i) Providing an aqueous solution of a protein source; (ii) Performing a continuous hydrolysis step wherein the aqueous solution of a protein source is treated with at least one endopeptidase by heating at 40 to 90°C and for a finite length of time of 2 to 20 minutes; (iii) heat inactivation of the endopeptidase. The invention also concerns a method of controlling viscosity and preventing bitter taste of a heat sterilized high protein composition, the method comprising providing an aqueous solution of a protein source and continuously hydrolyzing the aqueous solution of a protein source with at least one endopeptidase prior to heat inactivation of the said endopeptidase. Finally, the invention concerns the use of hydrolyzed protein prepared in a continuous process as described herein by treatment with at least one endopeptidase for a finite length of time, for preparing a heat sterilized composition and/or for controlling the viscosity of a liquid composition, wherein the composition comprises 5 to 20 weight % total protein. The invention also concerns medical uses and treatments applying or using the inventive heat sterilized composition.