Correction Lens System for Light Sheet Microscopy Aberrations
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Solution Overview
Problem
Light sheet microscopy techniques face limitations in axial resolution and sample preparation complexity due to thick light sheets and non-standard sample holders, and the use of a 45° configuration leads to imaging errors from oblique passage through separating layers, making high-throughput analysis and standard sample vessel compatibility challenging.
Innovation Solution
A correction lens system is integrated between the illumination/detection objectives and the separating layer system to correct aberrations caused by the oblique passage through the separating layer, allowing for the use of existing lenses and enabling easier adaptation to different cover glasses, reducing the number of lenses and manufacturing costs.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If a 45° configuration is used for illumination and detection objectives, then the light sheet can intersect the detection direction at a right angle enabling spatial recordings of thicker samples, but imaging errors occur due to oblique passage through separating layers
Solution Approach 1:
A correcting lens system is introduced as an intermediary element between the separating layer system and the detection objective. This correcting lens compensates for the aberrations caused by oblique light passage through the separating layer, thereby maintaining imaging accuracy while preserving the beneficial 45° configuration for thick sample imaging
Solution Approach 2:
The patent modifies the optical parameters by introducing a correcting lens system with specific focal length and positioning. By adjusting the lens parameters (focal length, distance from separating layer), the system compensates for aberrations and optimizes image quality while maintaining the 45° illumination-detection geometry
2Adaptability or versatility
If standard sample vessels with separating layer systems are used, then compatibility with existing sample holders is improved, but contamination risks increase and high-throughput analysis becomes challenging
Solution Approach 1:
The system separates the illumination/detection path from direct contact with the sample medium by using a separating layer system. This segmentation allows standard sample vessels to be used while preventing contamination, as the separating layer acts as a barrier between the optical system and the sample environment
Solution Approach 2:
The separating layer system serves as an intermediary barrier that enables the use of standard sample vessels while preventing contamination. The correcting lens system works in conjunction with this separating layer to maintain optical quality without requiring direct contact between objectives and sample medium
3Manufacturing precision
If correction lens systems are integrated to correct aberrations, then imaging resolution is enhanced, but device complexity increases
Solution Approach 1:
A correcting lens system is introduced as an intermediary element between the separating layer system and the detection objective. This correcting lens compensates for the aberrations caused by oblique light passage through the separating layer, thereby maintaining imaging accuracy while preserving the beneficial 45° configuration for thick sample imaging
Solution Approach 2:
The patent modifies the optical parameters by introducing a correcting lens system with specific focal length and positioning. By adjusting the lens parameters (focal length, distance from separating layer), the system compensates for aberrations and optimizes image quality while maintaining the 45° illumination-detection geometry
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The correction lens system enhances imaging resolution and compatibility with standard sample vessels, reducing contamination risks and enabling high-throughput analysis while maintaining image quality by correcting aberrations and allowing for the use of existing lenses.
Implementation Method 1
correction lens system with at least one correction lens, which is used to reduce such aberrations that occur as a result of the oblique passage of illumination light and/or light to be detected through the boundary surfaces of the separating layer system
Data Source
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AI summary
The invention relates to an assembly for light sheet microscopy. Said assembly comprises illumination optics having an illumination lens (5) for illuminating a sample (3), which is located on a sample carrier in a medium (2), wherein the sample carrier is aligned in respect of a flat reference surface (4), over an illumination beam path having a light layer, wherein the optical axis (6) of the illumination optics (5) and the light layer are in a plane which, together with the normal of the reference surface (4), includes an illumination angle (ß) different from zero, and detection optics having a detection lens (7) in a detection beam path having an optical axis (8) which, together with the normal of the reference surface (4), includes a detection angle (5) which is different from zero. The assembly furthermore comprises a separating layer system having at least one layer made of a specified material having a specified thickness which separates the medium (2) from the illumination lens (5) and the detection lens (7), wherein the separating layer system contacts the medium (2) together with a base area (9) aligned parallel to the reference surface (4), at least in the region accessible for the illumination lens (5) and the detection lens (7) for illumination or detection. The assembly finally also comprises a correction lens system having at least one correction lens (10, 11, 12) for reducing such aberrations which occur due to the oblique passage of illumination light and/or light to be detected through interfaces of the separating layer system. In an assembly according to the invention, the correction lens system is arranged between the illumination lens (5) and the separating layer system and/or between the detection lens (7) and the separating layer system.