Corynebacterium Strain Engineering for Higher L-Arginine Yield
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Solution Overview
Problem
Existing methods for producing L-arginine using microorganisms are limited in efficiency and yield, particularly in strains of the genus Corynebacterium, where the activity of specific proteins hinders optimal production.
Innovation Solution
A recombinant microorganism of the genus Corynebacterium with weakened activity of a protein having an amino acid sequence of SEQ ID NO: 1 is developed, achieved through genetic modification such as deletion or reduction of the corresponding polynucleotide, enhancing L-arginine production.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional Corynebacterium strains are used for L-arginine production, then the production process is simple, but the L-arginine yield is low
Solution Approach 1:
The patent removes the harmful argR gene from the Corynebacterium strain through genetic deletion. This extraction of the repressor gene eliminates the negative regulation mechanism that limited L-arginine production, thereby increasing yield without complicating the overall production system.
Solution Approach 2:
The patent changes the genetic parameter by deleting the argR gene, which alters the regulatory parameter of the bacterial strain. This genetic parameter change enables the strain to produce L-arginine at high levels while maintaining simplicity in the production process.
2Productivity
If protein activity is weakened to enhance L-arginine production, then L-arginine yield increases, but the microorganism's growth may be affected
Solution Approach 1:
The patent extracts and removes the argR repressor gene entirely from the microorganism's genome. This complete removal eliminates the negative regulatory function while preserving all other essential growth functions, thereby achieving high L-arginine production without compromising microorganism growth stability.
Data Source
AI summary
The present disclosure relates to an L-arginine-producing microorganism, in which a protein comprising an amino acid sequence of SEQ ID NO: 1 is weakened, and a method for producing L-arginine using the same.