Bacteria TRIZ Cases

Optimized antibody variable regions improve ILT4 binding while targeting MDSCs and TAMs to relieve tumor-microenvironment immunosuppression.

When oral delivery leaves respiratory protection uncertain, topical lactic acid bacteria induce interferon and activate dendritic cells for viral prophylaxis.

Bioactivity-HiTES uses small-molecule elicitors and supernatant screening to activate silent bacterial gene clusters without genetic manipulation.

Selected Lactobacillus strains combine vaginal-cell adhesion, hydrogen peroxide production, and acid-bile tolerance to target inconsistent probiotic benefits.

Low gas solubility and poor electron delivery constrain gas fermentation; modified hosts and fluidized-bed electrocatalysis improve mevalonate production.

ERV syncytin helps extracellular vesicles fuse with target-cell membranes, improving selective delivery while limiting immune response.

Modified fucosyltransferases help engineered microorganisms convert 3FL and 2'FL into LDFT while limiting by-product accumulation.

A low starting sulfur concentration plus dynamic sulfur feeding helps PHA-producing microorganisms improve productivity while using less sulfur.

Fecal bacterial measurements replace invasive biopsies and pathologist review for non-invasive canine IBD detection.

A Cellvibrio sp. KY-GH-1 enzyme hydrolyzes neoagarobiose into L-AHG and D-galactose under optimized conditions.

Weakening formate dehydrogenase activity in Corynebacterium redirects metabolism toward purine nucleotides while mitigating oxidative stress.

A divalent cation with pH adjustment above 11 stabilizes proteins in fermentation broth, improving recovery and retaining activity.

Plastic waste is degraded by Acinetobacter guillouiae repla2 into low-molecular-weight substances, supporting biological recycling of PET, PVC, PS, PP, and PE.

Controlled pH and lactic-acid-bacteria co-culturing improve Lacticaseibacillus paracasei survival under gastric and bile acid exposure.

Enhanced serine hydroxymethyltransferase activity helps microbial fermentation raise purine nucleotide yield despite cell membrane limits.

Slow, toxic lithium recovery is addressed with Glycomyces lithiumensis, which biologically concentrates lithium from substrates for LED-assisted harvesting.

Low-solubility ferric salt remains suspended with cultured bacteria, releasing Fe3+ for naked-eye Prussian blue detection without complex clay preparation.

Conventional renal treatments can cause constipation and dyspepsia; BP105 and BP121 probiotics remove uremic toxins and absorb phosphorus.

Enhanced glycine degradation supports purine nucleotide output while addressing low yield linked to membrane permeability and oxidative stress during fermentation.

A 16S rRNA-characterized EB-FPDK11 strain addresses inflammation, liver disease, and metabolic disorders while avoiding adverse side effects.

A defined Lactobacillus sakei HEM224 strain suppresses pro-inflammatory factors to address recurrent asthma inflammation.

Replacing corrosive chemical routes, the G180D/A205C nitrilase mutant converts the nitrile substrate at over 99% conversion in one-quarter the reaction time.

Replacing Salmonella O4 with APEC O78 and attenuating virulence factors helps RASVs target colibacillosis in poultry.

Non-sporulating Bacillus strains sequester toxic or unstable proteins in the sacculus, improving stability, recovery, and purification.

Genetically engineered Vibrio natriegens redirects carbon through anaerobic metabolism to produce succinate while reducing fossil dependence and emissions.

A composite microbial flora degrades tobacco components and produces aroma, shortening fermentation from years to 6–8 days.

Strain 1416G removes crown-gall functions while preserving T-DNA transfer to improve transformation of mature citrus tissues.

Multi-species microbial formulations biodegrade persistent glyphosate residues in contaminated substrates into less hazardous breakdown products.

Natural barcode cassettes distinguish nitrogen-fixing bacterial strains in mixed samples and quantify their relative colonization of plant roots.

Targeted amino acid mutations help transaminases process bulky substrates and stay stable during chiral amine synthesis.

Peptidyl transferase center mapping identifies E. coli ribosome mutants that expand sequence-defined polymer synthesis and support antibiotic resistance analysis.

Pre-acclimatizing anaerobic cultures with lactate and butyrate enriches caproate producers, stabilizing output despite complex microbial communities.

Engineered enzymes convert L-proline to trans-3-hydroxyproline with higher selectivity, supporting scalable production and simpler purification.

Defined Megasphaera strains produce butyrate to activate anti-tumor immunity while simplifying bacterial interaction screening.

Limited knowledge of fungal terpenoid genes is addressed by expressing Serendipita indica SiTPS in host cells to synthesize viridiflorol.

This case replaces toxic cyanide-based synthesis with recombinant fermentation, using oxidase and reductase steps to produce isopropanolamine.

Magnetic ionic liquids concentrate viable microbes before isothermal RPA, enabling field detection without thermal cyclers or powered tools.

Replacing costly sugar feedstocks, integrated ethane monooxygenase and metabolic pathways convert ethane to 3-hydroxypropionate in engineered microorganisms.

Specific bacterial genera identified through fecal microbiota transplantation address limited steroid efficacy in acute gut GVHD.

Low-yield cell-free phage production is addressed by modulating E. coli transcription and translation machinery to improve synthesis.

Targeted lysA promoter substitutions increase decarboxylase expression and raise L-lysine production in C. glutamicum by 2%–30%.

Removing the argR repressor weakens inhibitory protein activity in Corynebacterium and increases L-arginine yield for industrial production.

Methanotrophs and PHA-producing bacteria are combined to convert methane into biopolymer while reducing carbon loss as CO2.

A fluorescent protein probe replaces costly chromatographic or enzymatic assays with specific, quantitative monitoring of fructose-1,6-bisphosphate in living cells.

Conventional GMP fermentation can lose yield through weak XMP conversion and product degradation; CJG0497 addresses both bottlenecks.

Bacillus amyloliquefaciens ferments selected grains into an enzyme with α-amylase, protease, gluten-degrading, and fibrinolytic activities.

Because conventional treatments can miss hypoxic tumor cores, intratumoral C. novyi CFUs germinate there and liquefy solid tumors.

Hydrogenophilus transformants use chorismate-pyruvate lyase to produce 4-HBA from carbon dioxide instead of sugar-based feedstocks.

R97M and N43 substitutions improve L-proline-3-hydroxylase activity for L-pipecolic acid conversion, reaching up to 88% product yield.

Smob from Sulfuriflexus mobilis selectively fucosylates LNT for higher-purity LNFP-I production with minimal 2′-FL formation.