Corynebacterium CJG0497 Strain for High GMP Conversion

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Solution Overview

Problem

Current methods for producing 5'-guanosine monophosphate (GMP) face challenges in achieving high conversion efficiency and low degradation rates, necessitating the development of novel strains with improved GMP production capabilities.

Innovation Solution

The Corynebacterium ammoniagenes CJG0497 strain, deposited with Accession No. KCCM13320P, exhibits a high GMP conversion ability and low degradation rate, enabling efficient production of GMP through cultivation and recovery processes.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If conventional microorganisms are used for GMP production, then the production process can be maintained, but the conversion efficiency from XMP to GMP is insufficient and GMP degradation occurs

Engineering Contradiction:

ImproveGMP conversion efficiencyVSAvoidGMP degradation rate

Core Design Contradiction:

ProductivityVSReliability

Solution Approach 1:

The patent applies parameter changes by modifying the microbial strain's genetic parameters to achieve high GMP conversion efficiency and low degradation rate. Specifically, the Corynebacterium ammoniagenes CJG0497 strain has been genetically engineered with enhanced GMP synthase activity and suppressed GMP catabolic pathways, transforming the strain into an optimized production organism that resolves the contradiction between conversion efficiency and degradation resistance

Inventive Principle:

Principle #35Parameter changes

Solution Approach 2:

The patent extracts and eliminates harmful factors by removing or suppressing the genetic pathways responsible for GMP degradation in the microbial strain. Through gene deletion or inhibition of catabolic enzymes, the strain loses the ability to degrade GMP, thereby maintaining high GMP levels in the production medium while preserving conversion efficiency

Inventive Principle:

Principle #2Taking out (Extraction)

2Productivity

If existing microorganisms are used for GMP production, then the process is established, but high conversion ability and low degradation rate are not simultaneously achieved

Engineering Contradiction:

ImproveGMP production yieldVSAvoidGMP degradation

Core Design Contradiction:

ProductivityVSLoss of substance

Solution Approach 1:

The patent converts the previously harmful GMP degradation pathway into a beneficial feature by selectively eliminating it from the strain's metabolism. The Corynebacterium ammoniagenes CJG0497 strain has been engineered to lack functional GMP catabolic genes, transforming a potential loss mechanism into a complete preservation of GMP, thereby achieving high production yield with minimal substance loss

Inventive Principle:

Principle #22Blessing in disguise (Convert harm into benefit)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The strain achieves high-yield production of GMP, making it suitable for industrial applications by enhancing conversion from XMP to GMP and reducing degradation, thus overcoming existing inefficiencies.

Implementation Method 1

an enzyme reaction process which converts the fermentation product into GMP

Methodology Applied

Scientific EffectEnzymatic conversion: Enzyme

Implementation Method 2

a fermentation process which produces 5'-xanthosine monophosphate (XMP), an intermediate of the nucleic acid biosynthetic metabolic system, using microorganisms

Methodology Applied

Scientific EffectFermentation: Fermentation

Data Source

PatentEP4617360A1Strain for producing 5'-guanylic acid, and method for producing 5'-guanylic acid using same

Publication Date: 2025.09.17 CJ CHEILJEDANG CORP

AI summary

The present disclosure relates to a Corynebacterium ammoniagenes CJG0497 strain deposited with Accession No. KCCM13320P; a method for producing 5'-guanosine monophosphate (GMP), comprising culturing the strain in a medium; a composition for producing 5'-guanosine monophosphate (GMP), comprising the strain, a culture product of the strain, a fermented product of the strain, or a combination of two or more thereof; and use of the strain for the production of 5'-guanosine monophosphate (GMP).