Corynebacterium cbbL Deletion for L-Amino Acid Production

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Solution Overview

Problem

Existing methods for producing L-amino acids using Corynebacterium strains are not efficient, as they do not effectively enhance the production capabilities of these microorganisms.

Innovation Solution

A Corynebacterium microorganism with weakened activity of a protein comprising an amino acid sequence of SEQ ID NO: 1 is developed, which is cultured in a specific medium to enhance L-amino acid production.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If conventional Corynebacterium strains are used for L-amino acid production, then the production process is simple, but the productivity is low

Engineering Contradiction:

ImproveL-amino acid production efficiencyVSAvoidstrain modification complexity

Core Design Contradiction:

ProductivityVSDevice complexity

Solution Approach 1:

The patent removes the cbbL gene encoding ribulose-1,5-bisphosphate carboxylase/oxygenase from the Corynebacterium strain. This extraction of a specific gene that competes for carbon fixation redirects metabolic flux toward L-amino acid production, thereby improving productivity without requiring complex multi-gene modifications

Inventive Principle:

Principle #2Taking out (Extraction)

Solution Approach 2:

The patent modifies metabolic parameters by deleting the cbbL gene, which changes the carbon fixation pathway activity. This parameter change redirects carbon flow from the Calvin cycle toward amino acid biosynthesis pathways, enhancing L-amino acid production efficiency while maintaining cellular viability

Inventive Principle:

Principle #35Parameter changes

2Productivity

If gene expression is increased to enhance L-amino acid production, then productivity improves, but metabolic balance is disrupted

Engineering Contradiction:

ImproveL-amino acid production efficiencyVSAvoidmetabolic balance

Core Design Contradiction:

ProductivityVSStability of the object's composition

Solution Approach 1:

By removing the cbbL gene, the patent extracts the competing carbon fixation pathway, preventing metabolic imbalance that would arise from overexpressing amino acid biosynthesis genes. This approach maintains metabolic balance while redirecting resources to amino acid production

Inventive Principle:

Principle #2Taking out (Extraction)

Solution Approach 2:

Instead of increasing expression of amino acid biosynthesis genes to improve productivity, the patent inverts the approach by deleting a gene (cbbL) that competes for the same metabolic resources. This inversion resolves metabolic balance issues while achieving the same productivity goal

Inventive Principle:

Principle #13The other way round (Inversion)

Data Source

PatentUS20250327104A1L-amino-acid-producing corynebacterium sp. microorganism, and method for producing l-amino acids by using same

Publication Date: 2025.10.23 CJ CHEILJEDANG CORP

AI summary

Provided is a microorganism of the genus Corynebacterium producing L-amino acids and a method for producing L-amino acids using the same.