Mutant Preparation TRIZ Cases
- 15N-Labeled DNA Mutation by Proton Resonance Irradiation
15N-labeled DNA enables proton-beam mutation with 4.43 MeV gamma-ray feedback, quantifying local DNA damage and reducing screening time.
- Nanoscopic Projectile Delivery Through Atomic-Smooth Accelerator Channels
Atomic-smooth accelerator channels launch nanoscopic projectiles into target cells for localized drug delivery and genetic modification with less collateral damage.
- Bovine Embryonic Stem Cell Culture for Stable Pluripotency
A feeder-based medium with FGF2, Wnt inhibition, and optional ROCK inhibition improves bovine ESC derivation and preserves pluripotency.
- High-RNA Yeast Breeding via Adaptive Evolution and Flow Cytometry
Fluorescent RNA staining, adaptive evolution, and flow cytometry speed screening of yeast strains that can exceed 15% RNA content.
- Guide RNA/Cas Plant Genome Editing for Precise Targeted Insertion
Targeted guide RNA/Cas cleavage enables precise plant genome insertion or deletion, avoiding random transgene integration and supporting fertile edited plants.