Soybean Cultivar S130089 Breeding via Agrobacterium Transformation

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Solution Overview

Problem

The development of new soybean cultivars is a time-consuming process that requires precise planning and resource management, aiming to combine desirable traits such as higher seed yield, disease resistance, drought tolerance, and improved fatty acid profile, while existing methods are inefficient in achieving these goals.

Innovation Solution

The introduction of soybean cultivar S130089, which is developed through crossing and mutagenesis or transformation, incorporating traits like resistance to glyphosate herbicides, Phytophthora Root Rot, and soybean cyst nematode, along with improved fatty acid composition, using methods like microprojectile-mediated delivery and Agrobacterium-mediated transformation.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If traditional breeding methods are used to develop new soybean cultivars, then the process can combine desirable traits from parental germplasm, but the development process takes 6-12 years and is time-consuming

Engineering Contradiction:
Improvecombination of desirable traitsVSAvoidbreeding process duration
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent applies preliminary action by using mutagenesis and transformation techniques to pre-introduce desired traits into the soybean cultivar before traditional breeding begins. This allows the cultivar to already possess resistance traits and improved fatty acid profiles, significantly reducing the time required for trait combination that would otherwise take multiple generations of crossing and selection.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent uses mutagenesis agents and transformation vectors as intermediaries to transfer desired traits into the soybean cultivar. These intermediaries directly introduce resistance genes and fatty acid modification genes, bypassing the need for lengthy traditional crossing programs to achieve the same trait combination.

Inventive Principle:
Principle #24Intermediary (Mediator)

2Ease of manufacture

If traditional breeding methods are used, then resource management and planning can be maintained, but the efficiency of achieving breeding goals is insufficient

Engineering Contradiction:
Improveresource managementVSAvoidbreeding efficiency
Core Design Contradiction:
Ease of manufactureVSProductivity

Solution Approach 1:

The patent replaces the mechanical system of traditional crossing and phenotypic selection with biotechnological methods including mutagenesis and genetic transformation. This substitution dramatically increases breeding efficiency by directly modifying the genetic makeup of the cultivar rather than relying on slow, generation-by-generation selection processes.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent applies parameter changes by directly altering genetic parameters through mutagenesis and transformation. This allows precise modification of traits such as disease resistance and fatty acid composition, achieving breeding goals much faster than traditional methods while maintaining systematic resource management.

Inventive Principle:
Principle #35Parameter changes

3Loss of time

If advanced genetic techniques like microprojectile-mediated delivery and Agrobacterium-mediated transformation are used, then breeding process time is reduced, but the complexity of the development process increases

Engineering Contradiction:
Improvebreeding process durationVSAvoidgenetic technique complexity
Core Design Contradiction:
Loss of timeVSDevice complexity

Solution Approach 1:

The patent uses specialized vectors and agents as intermediaries to simplify the complex process of genetic modification. By employing well-established systems like Agrobacterium-mediated transformation and microprojectile delivery, the patent manages technical complexity through standardized protocols while achieving rapid trait introduction.

Inventive Principle:
Principle #24Intermediary (Mediator)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

Soybean cultivar S130089 exhibits improved yield, disease resistance, and altered fatty acid profile, enhancing agricultural productivity and nutritional value, while streamlining the breeding process through advanced genetic techniques.

Implementation Method 1

using methods like microprojectile-mediated delivery and Agrobacterium-mediated transformation

Methodology Applied
Scientific EffectMicroprojectile-mediated delivery:

Implementation Method 2

using methods like microprojectile-mediated delivery and Agrobacterium-mediated transformation

Methodology Applied
Scientific EffectAgrobacterium-mediated transformation:

Data Source

PatentUS9060481B1Soybean cultivar S130089
Publication Date: 2015.06.23 MONSANTO TECHNOLOGY LLC

AI summary

A soybean cultivar designated S130089 is disclosed. The invention relates to the seeds of soybean cultivar S130089, to the plants of soybean cultivar S130089, to the plant parts of soybean cultivar S130089, and to methods for producing progeny of soybean cultivar S130089. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar S130089. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar S130089, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar S130089 with another soybean cultivar.