Corynebacterium IMP Export Protein Mutations
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Solution Overview
Problem
Current methods for producing 5′-inosine monophosphate (IMP) through microbial fermentation face challenges in achieving high yields due to inefficient export of IMP by microorganisms, limiting its application in food and medical fields.
Innovation Solution
A microorganism of the genus Corynebacterium with enhanced activity of IMP export proteins, specifically ImpE1 and ImpE2, is developed to increase the export efficiency of IMP, thereby improving production yields.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional microbial fermentation methods are used for IMP production, then the production process is simple, but the IMP yield is low due to inefficient export
Solution Approach 1:
The patent changes the activity parameter of the IMP export protein by introducing mutations in the impE1 and impE2 genes. Specifically, multiple amino acid substitutions are made in the ImpE1 and ImpE2 proteins to enhance their export activity, thereby increasing IMP production yield without fundamentally changing the fermentation process
Solution Approach 2:
The patent creates modified versions of the endogenous impE1 and impE2 genes with enhanced export activity. These mutated genes are introduced into the Corynebacterium glutamicum strain to replace or supplement the wild-type genes, effectively copying and improving the export function
2Productivity
If IMP export protein activity is enhanced through genetic modification, then IMP export efficiency increases, but the complexity of microorganism preparation increases
Solution Approach 1:
The patent optimizes the amino acid sequence parameters of the ImpE1 and ImpE2 proteins by introducing specific mutations. These parameter changes enhance the proteins' export activity while maintaining their basic structure and function, achieving improved efficiency without excessive complexity in preparation
3Productivity
If multiple mutations are introduced in ImpE1 and ImpE2 proteins, then IMP export activity is significantly enhanced, but the risk of affecting protein stability increases
Solution Approach 1:
The patent carefully selects and combines multiple mutations in the ImpE1 and ImpE2 proteins, making incremental changes to amino acid residues that are likely to affect export activity while minimizing disruptions to the overall protein structure. This approach enhances export activity while maintaining protein stability
Solution Approach 2:
The patent employs screening and selection processes to identify mutant strains that exhibit both enhanced export activity and maintained stability. Through iterative optimization and selection, stable high-yielding strains are obtained from the mutated genes
Data Source
AI summary
The present disclosure relates to a microorganism of the genus Corynebacterium producing 5′-inosine monophosphate, with an enhanced activity of an IMP export protein; a method for preparing 5′-inosine monophosphate using the same; a composition for producing 5′-inosine monophosphate; and a method for increasing export of 5′-inosine monophosphate.