Non-Enzymatic Trichoderma Broth for Corynebacterium Small-Molecule Production
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Solution Overview
Problem
Existing methods for increasing small molecule production in Corynebacterium cultures are limited, and the use of Trichoderma fermentation by-products is hindered by enzymatic activity that can interfere with Corynebacterium growth.
Innovation Solution
Supplementing Corynebacterium growth medium with the non-enzymatic fraction of spent Trichoderma fermentation broth, obtained through filtration, heat treatment, or protease incubation, to enhance small molecule production without enzymatic interference.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If spent Trichoderma fermentation broth is added to Corynebacterium culture to increase small molecule production, then productivity is improved, but enzymatic activity in the broth interferes with Corynebacterium growth
Solution Approach 1:
The patent extracts and removes the enzymatic fraction from spent Trichoderma fermentation broth, utilizing only the non-enzymatic fraction to supplement Corynebacterium culture media. This separation eliminates harmful enzymatic interference while preserving beneficial non-enzymatic components that enhance small molecule production.
Solution Approach 2:
The patent introduces a processed intermediate substance (non-enzymatic fraction) derived from spent Trichoderma broth as a mediator between the original broth and Corynebacterium culture. This intermediate form maintains beneficial properties while eliminating harmful enzymatic activity through prior processing.
2Productivity
If whole spent Trichoderma fermentation broth is used to supplement Corynebacterium culture, then small molecule production increases, but the complexity of the system increases due to enzymatic activity
Solution Approach 1:
The patent extracts and removes the enzymatic fraction from spent Trichoderma fermentation broth, utilizing only the non-enzymatic fraction to supplement Corynebacterium culture media. This separation eliminates harmful enzymatic interference while preserving beneficial non-enzymatic components that enhance small molecule production.
Solution Approach 2:
The patent modifies the chemical and functional parameters of the spent broth by removing enzymatic components through processing. This parameter change transforms the broth from a complex enzymatic mixture into a simplified non-enzymatic supplement that maintains productivity benefits while reducing system complexity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The non-enzymatic fraction of spent Trichoderma broth significantly increases small molecule production in Corynebacterium cultures, demonstrated by enhanced glutamic acid and lysine yields, independent of enzymatic activity.
Implementation Method 1
The non-enzymatic fraction of spent Trichoderma fermentation broth is produced by heat treating whole or fractionated spent Trichoderma fermentation broth
Implementation Method 2
The non-enzymatic fraction of spent Trichoderma fermentation broth is produced by filtering whole or fractionated spent Trichoderma fermentation broth
Data Source
AI summary
Described are methods for increasing the production of small molecules in a submerged Corynebacterium culture by supplementing Corynebacterium growth medium with the non-enzymatic fraction of spent Trichoderma fermentation broth.