Corynebacterium Cytochrome C Enhancement for Stable L-Amino Acid Production
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Existing methods for producing L-amino acids, particularly L-lysine, in Corynebacterium strains face challenges in maintaining high production potential throughout the fermentation process due to various stress factors, leading to decreased microbial activity in the late phase.
Innovation Solution
Enhancing cytochrome C activity in Corynebacterium microorganisms by introducing a cytochrome C coding gene, either exogenous or by strengthening intrinsic activity, to improve L-amino acid production potential and maintain it throughout the fermentation process.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If Corynebacterium strains are used for L-amino acid production through fermentation, then L-amino acids can be produced, but the production potential decreases in the late phase of cultivation due to stress factors and oxygen depletion
Solution Approach 1:
The patent introduces a cytochrome c gene to modify the physiological state of the microorganism, changing its respiratory efficiency and ability to utilize ferric iron. This parameter change enables the bacteria to maintain high production potential throughout the cultivation period by improving oxygen utilization and reducing stress responses, thereby resolving the contradiction between initial productivity and long-term stability
Solution Approach 2:
Cytochrome c acts as an intermediary substance that mediates electron transfer in the respiratory chain and facilitates iron metabolism. By introducing this intermediary, the patent improves the microorganism's ability to cope with oxygen depletion and stress factors, maintaining stable production potential through the cultivation process
2Quantity of substance
If microbial mass increases during fermentation, then more L-amino acid production capacity is available, but oxygen depletion occurs leading to decreased cellular activity
Solution Approach 1:
The introduction of cytochrome c changes the respiratory parameters of the microorganism, improving oxygen uptake efficiency and electron transport. This enables the microbial population to maintain high cellular activity even at high cell densities where oxygen would normally be depleted, resolving the contradiction between quantity and productivity
Data Source
AI summary
Provided are a microorganism for producing L-amino acid, having increased cytochrome C activity, and an L-amino acid production method using the microorganism.