Bacteria TRIZ Cases

Transfer selected gut microbes to improve organism traits without lengthy breeding.

Multiple probiotics and postbiotics reduce inflammation in DSS-induced colitis.

This case separates E. coli growth from protein production by limiting nitrogen after induction to improve fibroin-like protein yield.

This case uses heat-stable Bacillus spores to break down anti-nutritional factors and improve poultry feed efficiency.

CRISPR-Cas10 targets and degrades phage DNA, helping homologous recombination introduce mutations in Staphylococcus isolates.

This case uses site-directed deoB and PrhtA mutations to overcome slow growth, byproducts, and low L-threonine yield.

Specific enzyme mutations enable asymmetric transamination with 82% yield and 99% e.e. for sitagliptin intermediates.

REXER, GENESIS, and directed conjugation enable genome-wide codon compression for viable prokaryotic cells.

This case engineers TnaA, mutant TrpB, FMO, and IacA pathways to scale methane-based indigo production while reducing pollution.

CRISPR editing removes ethanol formation and redirects glucose metabolism for efficient, high-purity D-lactic acid fermentation.

This case uses Lacticaseibacillus rhamnosus FNZ142 in ruminant feed to improve productivity while inhibiting methanogens.

This case links human genes and bacterial pathways to acne severity, enabling targeted monitoring and microbiome-restoring treatment.

Selected Bacillus strains use enzymatic biodegradation to reduce dyes, lignin, COD, and paper-wipe persistence in wastewater.

This case converts PET into terephthalate, ethylene glycol, and β-ketoadipic acid through engineered microbial pathways.

Cultivating Dysosmobacter welbionis enables causal testing for gut microbiota therapies.

Adaptive laboratory evolution and targeted mutations improve foreign-DNA uptake while preserving E. coli Nissle probiotic functionality.

Microorganisms generate oxidizing ions through a masked culture-liquid jet to etch precise patterns on irregular metal surfaces.

Enzymatic PHA depolymerization releases hydroxyalkanoate monomers that microorganisms reuse to produce new PHA.

This case uses Komagataeibacter sp. GUS3, isolated from white grape vinegar, to cultivate bacterial cellulose at 1.644±0.4016 g/L.

NAD, serum, and selective inhibitors support AP growth while limiting commensal overgrowth and improving colony purity.

An internal dispense assembly forms collection media on-demand, reducing contamination during fluid particle and CFU analysis.

This case uses Bacillus subtilis 1579 to convert quercetin into PCA, addressing poor absorption while supporting inflammation reduction.

High-throughput screening and recombination produce P450-BM3 variants with higher conversion and turnover across diverse organic substrates.

Mutant soluble methane monooxygenases address host expression limits while enabling short-alkane conversion for lower-cost production.

This recombinant-cell pathway converts (S)-reticuline to (R)-reticuline at nearly 100% without optical resolution.

Genetically modified Pseudomonas strains remove virulence factors and tune alginate enzymes for safer, more consistent production.

This biological reactor uses Methanothermobacter thermautotrophicus to convert CO2 and electricity into methane for energy storage.

This case combines cellulase production, hydrolysis, and fermentation to process low-cost lignocellulosic biomass with fewer steps.

Engineered virulence-attenuated Gram-negative bacteria use secretion effectors to deliver therapeutic proteins into cancer cells and trigger apoptosis.

This bioprocess uses aryl sulfotransferase and enhanced PAPS supply to reduce phenolic toxicity during large-scale production.

This case uses bacterial host cells and reverse-transcribed templates to engineer phages against antibiotic-resistant bacteria.

This case uses Geobacillus enzymes to hydrolyze and ferment unprocessed biomass into PHA without pretreatment.

Gut-colonizing microbes expressing tannin acyl hydrolase or gallate decarboxylase convert ellagitannins into more bioavailable urolithins.

This platform uses bacterial bioaccumulation and filtration to recover rare earths from low-grade electronic waste.

Independent quorum-sensing and lysis circuits regulate each strain while plasmid-stability elements support durable synthetic co-cultures.

Engineered pro-region sequences help Gram-positive strains increase protein secretion and production, with productivity up to 50% higher.

Intestinal bacteria convert sodium selenite into a selenium-rich feeding solution, improving larval selenium use while reducing toxicity.

This case reprograms E. coli metabolism to convert crude glycerol into acetyl-CoA and NADPH, increasing PHB yield for lower-cost production.

Multiple microbial fermentations followed by inactivation improve postbiotic shelf-life, stability, safety, and prebiotic capability.

Dynamic metabolic valves redirect E. coli growth and NADPH flux, enabling 200 g/L xylitol from xylose at 86% theoretical yield.

Starch-based carriers biodegrade after food waste treatment, reducing disposal waste.

A nitrogen-limited citrate culture activates nitrogen fixation, enabling bacteria to produce L-glutamic acid without Haber-Bosch ammonia.

This probiotic strain targets adipocyte differentiation and lipid metabolism to reduce body fat and improve metabolic disease.

This case engineers Cas12a-68 amino acid residues to recognize more PAM types while retaining the simple Cas12a architecture.

A supplement combines amino acids, N-acetylhexosamine, and cobalamin to support high-yield anaerobe culture.

This case uses reduced A1386/A2405 and enhanced minC/minD expression to enlarge cells and simplify PHA separation and collection.

This case uses cytochrome C enhancement in Corynebacterium to improve sugar use and preserve L-amino acid productivity late in fermentation.

Gene knockouts and pathway overexpression help recombinant E. coli produce L-tyrosine at 80.5 g/L in fermentation.

Tumor-colonizing bacteria use a synchronized lysis circuit to release nanobodies and toxins locally, supporting antitumor immunity.

A segmented poly(A) coding sequence preserves plasmid templates in E. coli while supporting co-transcriptional mRNA tail addition.