Heat-Stable Bacillus Isolate Compositions for Poultry Feed Enzyme Stability
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Solution Overview
Problem
Existing animal feed supplements, particularly biosynthetic enzymes, face instability during feed processing and in the animal gut, leading to inefficient processing of anti-nutritional factors, and are costly to produce, limiting their effectiveness in improving nutrient uptake and gut health.
Innovation Solution
Development of Bacillus isolates, specifically Isolate 46 and Isolate 40, which are heat-stable and produce multiple enzymes to break down anti-nutritional factors in feed, such as non-starch polysaccharides and oligosaccharides, improving nutrient absorption and reducing footpad dermatitis in poultry.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If biosynthetic enzymes are added to animal feed to break down anti-nutritional factors, then nutrient absorption is improved, but the enzymes denature during heat treatment and lose stability in the animal gut
Solution Approach 1:
The patent uses heat-stable Bacillus spores that can withstand feed processing temperatures, replacing the fragile biosynthetic enzymes. The spores are inexpensive to produce and, while they may not remain active indefinitely, they provide sufficient enzymatic activity during the critical digestion period in the animal gut, effectively serving as a disposable solution that solves the stability problem.
Solution Approach 2:
The patent fundamentally changes the physical and chemical parameters of the enzymatic supplement by using spore-forming Bacillus species instead of free enzymes. The spores have different thermal stability parameters, allowing them to survive heat treatment at temperatures up to 90-100°C, and different pH stability parameters, allowing them to remain viable in the acidic environment of the animal gut.
2Adaptability or versatility
If biosynthetic enzymes are used to process anti-nutritional factors, then feed ingredient flexibility is increased, but production and purification costs are high
Solution Approach 1:
The patent employs Bacillus spores that are inexpensive to produce through simple fermentation processes, eliminating the need for complex purification steps required for biosynthetic enzymes. The spores can be produced at scale with low input costs and do not require expensive purification infrastructure, directly addressing the manufacturing cost issue while maintaining feed ingredient flexibility.
Solution Approach 2:
The Bacillus spores contain multiple endogenous enzymes that are naturally produced within the spore structure, eliminating the need for external enzyme addition or complex purification processes. The spores self-contained enzyme systems provide the necessary digestive functions, reducing manufacturing complexity and cost while maintaining versatility in feed formulation.
3Object-affected harmful factors
If heat treatment is applied during feed processing to ensure safety, then pathogen elimination is improved, but added enzymes denature and lose activity
Solution Approach 1:
The patent uses heat-stable Bacillus spores that can withstand the heat treatment temperatures (90-100°C) required for pathogen elimination. The spores maintain their structural integrity and enzymatic potential through the heat treatment process, unlike free enzymes that would denature. After heat treatment, the spores germinate in the animal gut and provide the necessary enzymatic activity.
Solution Approach 2:
The patent changes the thermal stability parameter of the enzymatic supplement by using spore-forming organisms. The spore structure provides thermal protection to the embedded enzymes, allowing them to survive heat treatment temperatures that would otherwise denature free enzymes. This parameter change enables simultaneous achievement of pathogen elimination and enzyme activity preservation.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The Bacillus isolates enhance growth rate, feed efficiency, and carcass quality in poultry on low energy diets while reducing the incidence of footpad dermatitis, offering a cost-effective alternative to biosynthetic enzymes by maintaining stability during feed processing and in the gut.
Implementation Method 1
The isolate has been deposited on Jun. 12, 2020 at the Agricultural Research Culture Collection (NRRL) under the terms of the Budapest treaty as Accession number NRRL B-67957. The isolate is a Bacillus amyloliquefaciens.
Implementation Method 2
Recently, numerous biosynthetic enzymes have been marketed to break down non-digestible substrates in the feed.
Implementation Method 3
the approach suffers from a number of shortcomings. First, the biosynthetic enzymes may not be stable and thus denature during heat treatments applied during feed processing.
Data Source
AI summary
The present invention provides Bacillus isolates, as well as probiotic and animal feed compositions comprising said isolates, which may be included in the diet of poultry to improve growth performance with reduced energy diets. Methods of using these compositions to improve nutritional uptake or to reduce the incidence of footpad dermatitis in poultry are also provided.