Recombinant Aryl Sulfotransferase Processes for Soluble Phenolics
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
There is a need for large-scale biological production processes that enhance the productivity and solubility of phenolic compounds, such as p-coumaric acid and resveratrol, which are often toxic and poorly soluble in water, limiting their uptake by the body.
Innovation Solution
A biological process using recombinant host cells expressing polypeptides with aryl sulfotransferase activity to sulfate phenolic compounds, enhancing their solubility and bioavailability, involving the use of polypeptides with specific amino acid sequences or sequence identities, and optimizing the host cells with increased PAPS supply and product removal.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If phenolic compounds are produced at large scale through fermentation, then productivity is improved, but the compounds become toxic to producing organisms and limit further productivity
Solution Approach 1:
The patent applies this principle by converting the toxic phenolic compounds into less toxic sulfated phenolic derivatives through enzymatic sulfation. The sulfation process transforms the harmful substance into a beneficial product with improved solubility and reduced toxicity, allowing continued large-scale production without organism damage
Solution Approach 2:
The patent changes the chemical parameter of the phenolic compounds by introducing sulfate groups through enzymatic reaction. This parameter change (sulfation) fundamentally alters the properties of the compounds, reducing their toxicity and improving their solubility, thereby enabling continued high-productivity fermentation
2Productivity
If phenolic compounds are produced in large quantities, then productivity is improved, but solubility in water deteriorates making uptake by the body difficult
Solution Approach 1:
The patent changes the chemical parameter of phenolic compounds by sulfation, which introduces polar sulfate groups that significantly improve water solubility. This parameter change allows large quantities of the compound to remain dissolved and bioavailable, resolving the contradiction between high productivity and poor solubility
Solution Approach 2:
The patent creates composite molecules by combining phenolic compounds with sulfate groups to form sulfated phenolic derivatives. This composite structure integrates the beneficial properties of both components: the phenolic core provides the desired biological activity while the sulfate groups provide water solubility and bioavailability
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The process enables efficient large-scale production of sulfated phenolic compounds, improving their solubility and bioavailability, thereby overcoming toxicity and solubility limitations.
Implementation Method 1
the first recombinant host cell comprises a heterologous polypeptide having an aryl sulfotransferase activity
Data Source
AI summary
The present invention generally relates to the field of biotechnology as it applies to the production of aryl sulfates using polypeptides or recombinant cells comprising said polypeptides. More particularly, the present invention pertains to polypeptides having aryl sulfotransferase activity, recombinant host cells expressing same and processes for the production of aryl sulfates employing these polypeptides or recombinant host cells.