Fermentative 1,5-Diaminopentane Isolation via Alkaline Hydrolysis
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Solution Overview
Problem
Existing methods for isolating 1,5-diaminopentane (DAP) from fermentation broths using organic solvents are suboptimal in terms of yield and time efficiency, particularly in industrial-scale applications.
Innovation Solution
A process involving alkalization of the fermentation broth, followed by thermal treatment and extraction with a suitable organic extractant, which cleaves acetyl-DAP hydrolytically and enhances phase separation rates, particularly effective with complex nutrient media.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If organic solvent extraction is used to isolate DAP from fermentation broth, then DAP can be extracted from the broth, but the extraction step is too slow and the overall process is too time-consuming
Solution Approach 1:
The patent applies parameter changes by adjusting the pH of the fermentation broth to alkaline conditions (pH 10-14) before extraction. This parameter change modifies the chemical state of DAP and acetyl-DAP, enabling hydrolytic cleavage of acetyl-DAP to release additional DAP during the extraction process, thereby increasing overall extraction efficiency while maintaining reasonable processing time
Solution Approach 2:
The patent implements preliminary action by performing alkaline treatment and thermal processing of the fermentation broth before the extraction step. This preliminary action hydrolytically cleaves acetyl-DAP to liberate bound DAP, so that when extraction is performed, all DAP (both free and previously bound) is available for extraction, increasing yield without extending the actual extraction time
2Quantity of substance
If organic solvent extraction is used to isolate DAP from fermentation broths, then DAP can be extracted, but the yield of product of interest is suboptimal due to acetyl-DAP not being cleaved
Solution Approach 1:
The patent changes the pH parameter to alkaline conditions (pH 10-14) and applies thermal treatment, which triggers hydrolytic cleavage of acetyl-DAP. This parameter change converts acetyl-DAP into free DAP, significantly increasing the total DAP yield from the fermentation broth without requiring complex additional processing steps
Solution Approach 2:
The patent uses alkaline conditions as an intermediary mechanism that facilitates the hydrolytic cleavage of acetyl-DAP. The alkaline environment acts as a mediator that enables the conversion of acetyl-DAP to free DAP, which then becomes available for extraction by the organic solvent, thereby improving yield while maintaining process simplicity
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This process significantly increases the yield and reduces the time required for DAP isolation, improving the overall efficiency and suitability for industrial-scale production.
Implementation Method 1
DAP-comprising byproducts of the fermentation, in particular acetyl-DAP, were surprisingly found here to be cleaved hydrolytically with liberation of the product of interest
Implementation Method 2
extracted thereafter with a suitable organic extractant. DAP-comprising byproducts of the fermentation, in particular acetyl-DAP, were surprisingly found here to be cleaved hydrolytically with liberation of the product of interest. Another surprise was the finding that the rate of phase separation in the extraction step can be increased substantially
Data Source
AI summary
The invention relates to a method for isolating 1,5-diaminopentane (DAP) from DAP-containing fermentation broths, to a method for the fermentative production of DAP using said isolating method and to a method for producing DAP-comprising polymers by using the DAP isolated or fermatively produced DAPs according to said methods.