Decellularized Adipose Tissue Bioscaffold for Soft Tissue Augmentation
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Solution Overview
Problem
Current methods for soft tissue augmentation, such as autologous, allogenic, and alloplastic approaches, face limitations including unpredictable results, high costs, immunogenic reactions, and rapid resorption, necessitating repeated treatments and posing concerns for disease transmission and mechanical mismatch with native tissue.
Innovation Solution
A method for decellularizing adipose tissue to preserve its extracellular matrix with a well-preserved three-dimensional structure, involving enzymatic digestion and solvent extractions, which results in a bioscaffold that can be used for soft tissue augmentation, cell culture substrates, and wound healing applications.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If free fat transfer is used for soft tissue augmentation, then autologous tissue is transplanted with available reserves, but graft resorption occurs due to lack of supporting vasculature leading to unpredictable results
Solution Approach 1:
The patent extracts and removes cellular components from adipose tissue through decellularization processes, isolating the extracellular matrix while eliminating immunogenic cells. This extraction approach creates a biocompatible scaffold that avoids rejection while preserving the structural framework needed for tissue regeneration.
Solution Approach 2:
The patent applies parameter changes by treating adipose tissue with enzymes (collagenase, dispase) and solvents to alter its physical and chemical properties. These treatments transform the tissue from a cellular state to a decellularized matrix state, changing porosity, composition, and structural characteristics to achieve the desired scaffold properties.
2Reliability
If vascularized flaps incorporating skin, fat, and muscle are used, then superior augmentation results are achieved, but donor site morbidity, deformity, and surgical time increase
Solution Approach 1:
The patent extracts only the essential extracellular matrix framework from adipose tissue, separating it from the complex cellular components. This isolation creates a simplified scaffold that provides structural support without requiring the complexity of vascularized flaps or causing donor site morbidity.
Solution Approach 2:
The decellularized extracellular matrix acts as an intermediary between the patient's own tissue and the implantation site. This intermediate scaffold provides a biocompatible framework that facilitates natural tissue regeneration without requiring complex donor site harvesting procedures.
3Ease of manufacture
If allogenic materials such as animal-derived collagen are used, then injectable bulking agents are available, but hypersensitivity, immune reactions, and disease transmission risks occur
Solution Approach 1:
The patent extracts and removes all cellular and immunogenic components from adipose tissue, leaving only the acellular extracellular matrix. This extraction eliminates proteins and cells that could trigger immune responses or transmit diseases, while preserving the structural framework.
Solution Approach 2:
The patent uses the patient's own adipose tissue as the source material, making the process autologous rather than allogenic. The patient's tissue serves itself as the material source, eliminating the need for animal-derived collagens or other allogenic materials that carry immunogenicity and disease transmission risks.
4Duration of action of stationary object
If decellularized human cadaveric dermis is used, then non-immunogenic material with extended duration is obtained, but resorption occurs requiring overcorrection and repeated treatments
Solution Approach 1:
The patent applies parameter changes through enzymatic and solvent treatments that modify the extracellular matrix structure. These treatments optimize porosity, cross-linking, and compositional parameters to enhance the scaffold's resistance to resorption while maintaining biocompatibility.
Solution Approach 2:
The patent creates a composite structure by combining the decellularized extracellular matrix with the patient's own regenerating tissue. This composite approach provides both immediate structural support and long-term integration, reducing resorption compared to using allogenic materials alone.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The decellularized adipose tissue provides a biocompatible scaffold that supports natural cellular processes, promotes adipogenesis, and maintains mechanical integrity, offering a durable and effective solution for soft tissue augmentation and regeneration without the need for repeated treatments.
Implementation Method 1
subjecting the adipose tissue to one or more incubations in an enzymatic digestion solution containing one or more enzymes
Implementation Method 2
one or more solvent extractions
Data Source
AI summary
This invention provides a method for decellularizing adipose tissue, comprising subjecting the adipose tissue to one or more incubations in an enzymatic digestion solution containing one or more enzymes, and one or more solvent extractions, wherein decellularized adipose tissue comprising an extracellular matrix with well-preserved three-dimensional structure is obtained. The invention also provides a decellularized adipose tissue comprising an extracellular matrix with well-preserved three-dimensional architecture, and bioscaffolds, microcarrier beads, and coatings comprising the decellularized adipose tissue.