Decellularized Skin Tissue Hydrogel for Artificial Skin
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current artificial skin prepared from collagen hydrogel is fragile, loses volume over time, and fails to replicate the dermal layer composition of real skin, limiting its similarity to natural skin.
Innovation Solution
A method for decellularizing skin tissue using trypsin and EDTA solutions, followed by DNA treatment and cell removal steps, to create a hydrogel that mimics the composition and structure of real skin, incorporating normal human dermal fibroblasts and epithelial keratinocytes, and dissolving decellularized skin tissue with pepsin in acetic acid to form a pre-gel.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of manufacture
If collagen hydrogel is used to prepare artificial skin, then the preparation process is simple, but the artificial skin becomes fragile with weak physical properties
Solution Approach 1:
The patent uses decellularized skin tissue as a composite material that contains collagen, elastin, glycosaminoglycans, and other extracellular matrix components together. This composite structure provides both the ease of hydrogel preparation and the mechanical strength of multiple structural proteins, resolving the contradiction between simple preparation and strong physical properties.
Solution Approach 2:
The patent changes the material parameters from pure collagen hydrogel to decellularized skin tissue hydrogel, which alters the compositional parameters to include multiple extracellular matrix components. This parameter change transforms the material properties to achieve both ease of preparation and enhanced mechanical strength.
2Ease of manufacture
If collagen hydrogel is used to prepare artificial skin, then the preparation is straightforward, but the artificial skin does not replicate the dermal layer composition of real skin
Solution Approach 1:
The decellularized skin tissue serves as a composite material containing the full spectrum of dermal extracellular matrix components including collagen, elastin, glycosaminoglycans, and other proteins. This composite composition replicates the complex dermal layer structure of real skin while maintaining the straightforward hydrogel preparation approach.
Solution Approach 2:
The patent copies the natural dermal extracellular matrix composition by using decellularized skin tissue, which preserves the authentic biochemical composition and structural organization of real skin dermis. This copying approach achieves high similarity to real skin without complicating the preparation process.
3Duration of action of moving object
If artificial skin is incubated for a long period of time, then cell culture can be performed, but the volume decreases significantly and cells die
Solution Approach 1:
The patent changes the hydrogel matrix parameters from collagen-only to decellularized skin tissue, which alters the biochemical environment parameters to include multiple extracellular matrix components. This parameter change improves the hydrogel's structural stability and biochemical support, enabling long-term cell culture with maintained cell viability and reduced volume loss.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method produces artificial skin with enhanced strength and durability, maintaining structural components like collagen, glycosaminoglycans, and hyaluronic acid, closely resembling real skin, and supports better cell growth and skin barrier function.
Implementation Method 1
a peeling preparation step of treating the skin tissue with a first solution containing trypsin
Implementation Method 2
The first solution may further include ethylenediaminetetraacetic acid (EDTA)
Implementation Method 3
a DNA treatment step in which the skin tissue, after undergoing the peeling step, is treated using a second solution that includes magnesium ions and DNase
Implementation Method 4
a cell removal step in which the skin tissue, after undergoing the peeling step, is treated using a buffer solution that includes ethylenediaminetetraacetic acid (EDTA) and a non-ionic surfactant
Implementation Method 5
dissolving decellularized skin tissue with pepsin in acetic acid to form a pre-gel
Implementation Method 6
The decellularized skin tissue according to an embodiment of the present invention to achieve the above objects may be a decellularized skin tissue which is lyophilized for at least 40 hours
Data Source
AI summary
A method for decellularization of a skin tissue according to an embodiment of the present invention comprises: a step of preparing a skin tissue to be decellularized; a peeling preparation step of treating the skin tissue with a first solution containing trypsin; and a peeling step of removing subcutaneous fat from the skin tissue after the peeling preparation step.