Decellularized Tissue Heat-Ratio Control for Cell Adhesion
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Decellularized tissues used in regenerative medicine exhibit variations in host cell adhesiveness, infiltration rate, and tissue regeneration due to differences in decellularization methods, leading to potential rejection and reduced effectiveness.
Innovation Solution
A decellularized tissue with a specific ratio of endothermic heat before and after decellularization, as measured by differential scanning calorimetry, is developed to enhance cellular adhesiveness, cell attraction, and differentiation-inducing effects.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional decellularization methods are used to remove cells from living body tissue, then the DNA content decreases and graft rejection is reduced, but the cellular adhesiveness and tissue regeneration capability deteriorate
Solution Approach 1:
The patent applies parameter changes by controlling the decellularization process to maintain the endothermic heat ratio within a specific range (0.8-1.2), which corresponds to optimal DNA removal while preserving extracellular matrix integrity. This quantitative parameter control resolves the contradiction between reducing DNA content for rejection resistance and maintaining structural properties for cellular adhesiveness
Solution Approach 2:
The patent creates a composite structure by preserving the extracellular matrix (collagen, elastin, proteoglycans) while removing cellular components. The resulting decellularized tissue maintains the natural composite architecture of living tissue, providing both the low immunogenicity of acellular material and the bioactive signals needed for cell adhesion and regeneration
2Quantity of substance
If different decellularization methods are used, then the degree of cell removal varies, but significant differences arise in host cell infiltration rate and tissue regeneration
Solution Approach 1:
The patent introduces feedback control by using endothermic heat measurement as a quantitative indicator to monitor the decellularization process. By measuring the endothermic heat ratio and adjusting decellularization conditions accordingly, the method achieves optimal cell removal while maintaining tissue regeneration capability, preventing over-decellularization that would harm regeneration
Solution Approach 2:
The patent replaces conventional mechanical or chemical assessment methods with thermal analysis (differential scanning calorimetry) to evaluate decellularization effectiveness. This substitution provides a more sensitive and accurate measurement of tissue structural integrity, enabling precise control over the balance between cell removal and regeneration preservation
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The decellularized tissue demonstrates low cytotoxicity, high cell attraction, and high tissue-regenerative effects, promoting rapid and effective tissue regeneration.
Implementation Method 1
a decellularized tissue showing a small change in the amount of endothermic heat before and after decellularization of a living-body derived tissue... can promote rapid tissue regeneration
Data Source
Figure 1
Figure 2
Figure 3
AI summary
The present invention addresses the problem of providing a decellularized tissue having excellent cell adhesion properties. Provided is a decellularized tissue prepared by decellularizing a biological tissue, said decellularized tissue being characterized in that the ratio of endothermic energy amount, in terms of dry mass, of the decellularized tissue to the biological tissue before the decellularization is 0.70 or greater when measured by differential scanning calorimetry. The ratio by mass of DNA amount, in terms of dry mass, of the decellularized tissue to the biological tissue before the decellularization may be 0.300 or smaller.