Decellularized Tissue Composition with Protein A for Regeneration
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Existing decellularized tissues used in regenerative medicine face challenges with graft rejection and poor tissue compatibility, leading to issues such as dropout and infection at the joint site between the graft and living tissue.
Innovation Solution
A decellularized tissue composition containing specific proteins with molecular weights between 30,000 to 70,000 and isoelectric points of 6.00 to 9.00, along with proteins of 3,000 to 15,000 and isoelectric points of 3.00 to 5.50, enhances tissue strength and promotes good tissue regeneration.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If decellularized tissue is used to avoid graft rejection, then biocompatibility is improved, but tissue strength and structural integrity deteriorate
Solution Approach 1:
The patent applies parameter changes by controlling the molecular weight range (30,000-70,000) and isoelectric point range (pI 6.00-9.00) of protein A, as well as the molecular weight range (3,000-15,000) and isoelectric point range (pI 3.00-5.50) of protein B. These specific parameter specifications optimize both the biocompatibility and mechanical strength of the decellularized tissue composition, resolving the contradiction between softness and strength.
Solution Approach 2:
The patent uses composite materials by combining decellularized tissue with specific proteins (protein A and protein B) having defined molecular weights and isoelectric points. This composite approach enhances the mechanical properties and cell adhesiveness of the decellularized tissue while maintaining its biocompatibility, thereby simultaneously improving both reliability and strength.
2Reliability
If decellularized tissue is used to promote tissue regeneration, then cell adhesiveness is improved, but handling performance deteriorates
Solution Approach 1:
The patent applies parameter changes by specifying the molecular weight (30,000-70,000) and isoelectric point (pI 6.00-9.00) of protein A, and molecular weight (3,000-15,000) and isoelectric point (pI 3.00-5.50) of protein B. These parameter optimizations enhance cell adhesiveness and tissue regeneration ability while maintaining appropriate tissue strength for surgical handling, thus resolving the contradiction between regeneration capability and ease of operation.
Solution Approach 2:
The patent employs composite materials by integrating decellularized tissue with specifically characterized proteins (protein A and protein B). This composite structure provides both excellent cell adhesiveness for tissue regeneration and sufficient mechanical strength for surgical handling, simultaneously improving reliability and ease of operation.
3Reliability
If artificial tissues are used to solve graft rejection, then compatibility with living tissues is improved, but dropout and infection at joint sites occur
Solution Approach 1:
The patent applies parameter changes by defining specific molecular weight (30,000-70,000) and isoelectric point (pI 6.00-9.00) ranges for protein A, and molecular weight (3,000-15,000) and isoelectric point (pI 3.00-5.50) ranges for protein B. These parameter specifications create a tissue composition that is highly compatible with living tissues while maintaining structural integrity to prevent dropout and infection at joint sites, thereby resolving the contradiction between compatibility and harmful effects.
Solution Approach 2:
The patent uses composite materials by combining decellularized tissue with specifically characterized proteins (protein A and protein B). This composite structure enhances biocompatibility while providing sufficient mechanical strength and structural integrity to prevent dropout and infection, simultaneously improving reliability and reducing harmful factors.
Data Source
AI summary
The object of the present invention is to provide a decellularized tissue that has appropriate strength and exhibits good tissue regeneration.The problem can be solved by a decellularized tissue composition comprising a decellularized tissue and a protein A with (a) molecular weight of 30,000 to 70,000 and (b) isoelectric point of pI 6.00 to 9.00