Microorganism Lysis TRIZ Cases

Piezoelectric MEMS and microfluidic lysis applies pulsed high-stress tips to break tough microbial cell walls for single-cell RNA-seq.

Multiple shear steps lyse microbial cells in oil to achieve high cell loading, stable particle size, and safer omega-3 storage and transport.

Dewatering fermentation broth before 60-110°C demulsification cuts solvent and salt use while improving microbial PUFA oil recovery.

Controlled osmotic shock and acidification extract periplasmic peptides at scale while preserving internal membranes and improving purity.

Staged shear lysis in oil enables high-solids microbial suspensions with better oxidative stability and lower transport volume for feed use.

Acid treatment and diafiltration remove vaccine polysaccharide impurities while controlling molecular weight and preserving high recovery.

Eliminating centrifugation-based clarification lets unclarified cellular lysates support protein synthesis while reducing processing time, energy, and equipment needs.

Automated lysis, selective capture, and purification in single-use cartridges shorten nucleic acid workflows and limit contamination.

Direct lysis with proteinase K inactivates RNases to stabilize RNA, eliminating centrifugation steps that increase processing time.

A functionalized nonwoven filter captures cells and disrupts them via osmotic pressure changes to release intracellular products.

Segmented physical and enzymatic processing isolates high-purity yeast biomolecules while reducing energy consumption.

Spray pressurized solvent into a low-pressure zone to rupture cell membranes, eliminating heat damage from mechanical friction.

Altering N-glycosylation pathways in Yarrowia lipolytica reduces mechanical disruption energy while maintaining cell integrity during fermentation.

Optimized domiphen bromide concentrations precipitate host cell DNA while preserving adenovirus particles, resolving yield losses from high contaminant loads.

Low-pH separation isolates RNA in cell walls, boosting ribonucleotide yield while removing turbidity-causing yeast components.

A rotor-stator system disrupts cells using controlled shear stress to release intracellular materials.

A decellularized tissue composition incorporates protein A with specific molecular weight and isoelectric point ranges to enhance cell adhesiveness.

A photocleavable polymer reversibly fixes cells to enable selective lysis via irradiation.

Salting precipitation removes host cell proteins, eliminating ultracentrifugation toxicity.

A rotating disc apparatus uses a planetary gear mechanism to maintain constant vial orientation during automated sample lysis.

Anionic surfactant lysis buffers keep DNA in solution, while size-exclusion chromatography removes contaminants to reduce mechanical degradation.

A cell-substrate impedance monitoring device tracks electrical resistance changes to quantify cytolytic activity of immune cells on cancer targets.

Chemical lysis replaces mechanical homogenization to isolate high-purity mitochondria, reducing nuclear DNA contamination.

Engineered oleaginous bacteria use inducible lysis to release lipids, eliminating costly sterilization and solvent extraction steps.

A serial cellular analytic system integrates sequential separation and controlled on-chip lysis to process extracellular, cytoplasmic, and nucleic fluids within a single device.

Alkaline thermal lysis extracts squalene from yeast cells, preventing emulsions and reducing material consumption.

An electrophoretic decellularization device removes cellular material from tissue using an electric potential across electrodes.

Nebulization atomizes microbial suspensions to achieve rapid thermal lysis, reducing energy consumption compared to conventional high-pressure methods.

Ion concentration polarization generates electroconvective vortices to mechanically shear bacterial cells using low electric fields.

Self-generated biosolvent extracts microbial lipids to bypass lignin inhibition and boost sugar conversion yields.

Fluidized solid supports in a microfluidic cartridge reduce pressure drops and bubble formation during nucleic acid extraction from small cell counts.

Pre-incubating mycobacteria with organic solvents or zinc chloride weakens cell walls, resolving low DNA extraction efficiency caused by robust structures.

Proprietary GMP reagent set optimizes serum-free suspension culture to deliver up to 2×10^11 viral genomes per milliliter.