Serial Cellular Analytic System for Contamination-Free Multi-Fluid Analysis
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Solution Overview
Problem
Current methods for analyzing cellular fluids, such as extracellular, cytoplasmic, and nucleic fluids, are inefficient and prone to contamination, as they often require separate samples and systems, leading to loss of valuable information and increased complexity and cost.
Innovation Solution
A serial cellular analytic system that uses sequential separation and controlled on-chip lysis to analyze different cellular components, including extracellular, cytoplasmic, and nucleic fluids, within a single integrated device, allowing for simultaneous and automated analysis of various fluids while maintaining their separation to prevent contamination.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If separate samples and systems are used to analyze different cellular fluids, then contamination is reduced, but device complexity and operational complexity increase
Solution Approach 1:
The patent combines multiple analysis functions for different cellular fluids (extracellular, cytoplasmic, nucleic) into a single integrated device. The device includes multiple separation chambers and analysis chambers that can process different fluid types simultaneously within one system, eliminating the need for separate samples and systems while maintaining contamination prevention through physical separation within the device.
Solution Approach 2:
The device is segmented into distinct functional modules including separate separation chambers for different cell types and corresponding analysis chambers. Each chamber is designed to handle specific cellular fluids independently, allowing simultaneous analysis while preventing cross-contamination through physical isolation of each analysis pathway within the integrated device.
2Reliability
If separate samples and systems are used for analyzing cellular fluids, then contamination is minimized, but information loss increases due to inability to analyze multiple fluids from single sample
Solution Approach 1:
The integrated device enables simultaneous analysis of multiple cellular fluids (extracellular, cytoplasmic, and nucleic) from a single sample by combining multiple separation and analysis functions in one system. This allows comprehensive information collection from all fluid types without the need for separate sampling, thereby preventing information loss while maintaining contamination prevention through internal separation mechanisms.
3Ease of manufacture
If manual separation and analysis methods are used, then equipment cost is reduced, but productivity decreases and contamination risk increases
Solution Approach 1:
The device incorporates automated sample processing capabilities where the system performs separation and analysis operations automatically without requiring extensive manual intervention. The integrated design allows the device to handle multiple fluid types through programmed sequences, improving productivity and reducing contamination risk from manual handling while maintaining cost-effectiveness through a single-device solution.
4Measurement precision
If multiple separate systems are used to analyze different cellular fluids, then analysis accuracy is maintained, but operational complexity and costs increase
Solution Approach 1:
The patent integrates multiple analysis functions for different cellular fluids into a single device with standardized operating procedures. The device maintains measurement precision for each fluid type through dedicated separation and analysis chambers while reducing operational complexity by providing a unified interface and automated processing sequences, eliminating the need to operate multiple separate systems.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach automates the analysis of multiple fluids, enhances the collection of information, reduces sample requirements, and minimizes contamination, resulting in more accurate and reliable data with reduced operational complexity and costs.
Implementation Method 1
a separator to separate a cellular particle from a surrounding fluid
Implementation Method 2
at least one lysing device coupled to at least a second outlet of the separator to rupture a membrane of the cellular particle
Data Source
AI summary
In one example in accordance with the present disclosure, a cellular analytic system is described. The cellular analytic system includes a series of analytic devices. Each analytic device includes 1) a separator to separate a cellular particle from a surrounding fluid, 2) an analyzer coupled to a first outlet of the separator to analyze the surrounding fluid, and 3) at least one lysing device coupled to at least a second outlet of the separator to rupture a membrane of the cellular particle. An outlet of the lysing device is fluidly coupled to a separator of a downstream analytic device.