Rotor-Stator Cell Disruption for PUFA Bioavailability
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Solution Overview
Problem
The challenge lies in the instability of polyunsaturated fatty acids (PUFAs) against oxidative degradation when isolated from cells, and the harsh conditions required for cell disruption often damage these valuable materials during the production of feed or food, leading to inadequate bioavailability and material damage.
Innovation Solution
A rotor-stator system is used for cell disruption with controlled energy input, maintaining a solids content of 30-70% and energy input up to 50 kWh/t, along with antioxidants and optional enzymes to stabilize and release PUFAs gently, ensuring effective cell disruption and minimizing damage.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If harsh cell disruption conditions with high mechanical energy input are used to achieve satisfactory cell disruption, then cell disruption rate is improved, but valuable substances (PUFAs) are damaged due to oxidative degradation
Solution Approach 1:
The patent changes the key parameter of mechanical energy input from high levels (harsh conditions) to low levels (maximum 50 kWh/t, preferably 5-20 kWh/t), combined with specific solids content control (30-70 wt.%), to achieve effective cell disruption while minimizing oxidative degradation of PUFAs
Solution Approach 2:
The patent introduces antioxidants as intermediary substances that mediate between the cell disruption process and the PUFAs, protecting the PUFAs from oxidative degradation caused by mechanical energy input during the disruption process
2Object-affected harmful factors
If cell disruption is insufficient to maintain PUFAs stable, then oxidative degradation is reduced, but bioavailability of PUFAs is reduced because cell wall cannot be broken down
Solution Approach 1:
The patent optimizes parameters including solids content (30-70 wt.%, preferably 45-65 wt.%) and mechanical energy input (5-20 kWh/t) to achieve the optimal balance between cell disruption effectiveness and PUFA stability, ensuring both bioavailability and protection from degradation
3Productivity
If high mechanical energy input is applied to achieve cell disruption, then cell disruption is improved, but valuable substances are damaged
Solution Approach 1:
The patent fundamentally changes the energy input parameter from high to low (maximum 50 kWh/t, preferably 5-20 kWh/t), combined with high solids content (30-70 wt.%), to achieve effective cell disruption while maintaining the stability and integrity of valuable substances
Solution Approach 2:
Antioxidants are introduced as intermediary substances that protect valuable substances from the harmful effects of mechanical energy input, allowing effective cell disruption to occur without damaging the valuable substances
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method achieves significant reduction in mechanical energy input while maintaining high cell disruption rates and stabilizing PUFAs, ensuring their bioavailability and minimizing oxidative degradation, resulting in a more efficient and gentle process for producing feed or food containing valuable lipids.
Implementation Method 1
In this gap, the cells are subjected to shear stress, and turbulence is also generated. These two factors cause cell disruption.
Implementation Method 2
In this gap, the cells are subjected to shear stress, and turbulence is also generated. These two factors cause cell disruption.
Implementation Method 3
A problem with the use and processing of numerous valuable substances, especially polyunsaturated fatty acids, is their susceptibility to oxidative degradation
Data Source
AI summary
According to the invention, a rotor-stator system allows cells containing useful materials to be disrupted in a very gentle manner.