1,5-Diaminopentane Purification via pH-Driven Salt Inversion
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Solution Overview
Problem
Current methods for refining 1,5-diaminopentane from fermentation broth have low yields and result in the compound being obtained as a salt, requiring additional processing to obtain pure 1,5-diaminopentane.
Innovation Solution
A method involving the concentration of a fermentation broth containing a carbonate of 1,5-diaminopentane, followed by the addition of an acid to create an acidic composition with a pH of 4 to 7, and then a base to produce a basic composition with a pH of 12 to 14, allowing for the recovery of high-purity 1,5-diaminopentane.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If 1,5-diaminopentane is prepared by removing microorganisms from lysine fermentation broth and using lysine decarboxylase, then 1,5-diaminopentane can be obtained, but the yield is low and additional salt removal processes are required
Solution Approach 1:
The patent applies parameter changes by adjusting pH levels through acid and base addition to transform 1,5-diaminopentane between its free base and salt forms. This enables selective precipitation and separation of the product from the fermentation broth, directly achieving high purity 1,5-diaminopentane with high yield while eliminating the need for multiple salt removal steps
Solution Approach 2:
The patent performs preliminary concentration of the fermentation broth before pH adjustment and product recovery. This preliminary action removes excess water and concentrates the 1,5-diaminopentane, improving subsequent separation efficiency and overall yield while reducing the number of additional processing steps required
2Manufacturing precision
If 1,5-diaminopentane is obtained in the form of a salt, then it can be recovered as a crystal, but an additional process is required to remove the salt and obtain pure 1,5-diaminopentane
Solution Approach 1:
The patent applies inversion by first converting 1,5-diaminopentane to its salt form for easy crystallization and recovery, then inverting the process by adding base to convert the recovered salt back to the free base form. This two-step inversion approach enables both high purity recovery through crystallization and high overall yield by recovering the product in salt form before converting to free base
Solution Approach 2:
The patent uses parameter changes in pH to control the form of 1,5-diaminopentane. By adjusting pH to acidic conditions, the free base converts to salt form for crystallization. After recovery, adding base changes the pH to convert the salt back to free base form, achieving both high purity and high yield
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enables the attainment of high-purity 1,5-diaminopentane with a high yield by effectively removing carbonate and separating 1,5-diaminopentane from its carbonate form, facilitating its recovery.
Implementation Method 1
adding an acid to a concentrate of the fermentation broth to prepare a carbonate removed acidic composition
Implementation Method 2
adding a base to the acidic composition to prepare a basic composition with a pH of about 12.0 to about 14
Implementation Method 3
concentrating a fermentation broth including a carbonate of 1,5-diaminopentane
Data Source
Figure 1~2
AI summary
Provided are a method of refining a reaction solution including a carbonate of 1,5-diaminopentane, which is produced by fermentation, through a decarbonation step and a pH adjustment step, and 1,5-diaminopentane which is refined by the above method.