Dishwashing Detergent Enzyme Pairing for Low-Temperature Stain Removal

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Solution Overview

Problem

Modern dishwashing detergents struggle to maintain high cleaning performance on stubborn stains at lower washing temperatures, leading to consumer dissatisfaction and increased water and energy consumption due to pre-treatment of stains.

Innovation Solution

A hand dishwashing detergent containing a specific combination of amylase and protease, where the amylase has an amino acid sequence identical to SEQ ID NO:1 and the protease has an amino acid sequence identical to SEQ ID NO:3, synergistically improves cleaning performance on enzyme-sensitive soils, particularly at lower temperatures.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Use of energy by stationary object

If lower washing temperatures are used, then energy consumption is reduced, but cleaning performance on stubborn stains deteriorates

Engineering Contradiction:
Improveenergy consumptionVSAvoidcleaning performance on stubborn stains
Core Design Contradiction:
Use of energy by stationary objectVSReliability

Solution Approach 1:

The patent changes the chemical parameters of the cleaning system by introducing specific enzyme combinations (amylase with specific activity ≥4000 U/mg and protease with specific activity ≥2000 U/mg) that maintain cleaning effectiveness at lower temperatures. This allows the washing process to operate at reduced temperatures while preserving cleaning performance through optimized enzymatic activity parameters.

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The invention uses a composite enzymatic system combining multiple enzymes (amylase and protease with specific activity ratios) to achieve enhanced cleaning performance at low temperatures. This composite approach leverages the synergistic effects of different enzymes targeting various stain components, maintaining reliability while reducing energy input.

Inventive Principle:
Principle #40Composite materials

2Use of energy by stationary object

If conventional detergents are used at lower temperatures, then energy consumption is reduced, but cleaning performance on enzyme-sensitive soils deteriorates

Engineering Contradiction:
Improveenergy consumptionVSAvoidcleaning performance on enzyme-sensitive soils
Core Design Contradiction:
Use of energy by stationary objectVSReliability

Solution Approach 1:

The patent optimizes the enzymatic parameters by selecting amylase with specific activity ≥4000 U/mg and protease with specific activity ≥2000 U/mg, creating a chemically optimized system that maintains high cleaning performance on enzyme-sensitive soils at lower temperatures without requiring conventional high-temperature processing.

Inventive Principle:
Principle #35Parameter changes

3Use of energy by moving object

If washing temperature is reduced, then energy consumption and water heating requirements are reduced, but cleaning effectiveness on stubborn soiling deteriorates

Engineering Contradiction:
Improvewater heating energyVSAvoidcleaning effectiveness on stubborn soiling
Core Design Contradiction:
Use of energy by moving objectVSReliability

Solution Approach 1:

The invention changes the chemical action parameters by employing enzymes with optimized specific activities that function effectively at lower temperatures. The amylase (≥4000 U/mg) and protease (≥2000 U/mg) maintain catalytic efficiency at reduced temperatures, enabling effective removal of stubborn soiling without the need for high water heating energy input.

Inventive Principle:
Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The enzyme combination significantly enhances the removal of stubborn dirt, such as starch-based soils, at temperatures below 60°C, providing improved cleaning performance without reducing effectiveness on other types of soiling.

Implementation Method 1

the amylase has an amino acid sequence that is 100% identical over its entire length to the amino acid sequence specified in SEQ ID NO:1

Methodology Applied
Scientific EffectEnzymatic hydrolysis: Enzyme

Implementation Method 2

cleaning performance on enzyme-sensitive soils, particularly at lower temperatures

Methodology Applied
Scientific EffectHydrolysis: Hydrolysis

Implementation Method 3

the at least one protease has the amino acid sequence according to SEQ ID NO:3

Methodology Applied
Scientific EffectEnzymatic hydrolysis: Enzyme

Implementation Method 4

cleaning performance on enzyme-sensitive soils

Methodology Applied
Scientific EffectHydrolysis: Hydrolysis

Implementation Method 5

the use of a combination of a specific protease with a specific amylase synergistically improves the cleaning performance of corresponding hand dishwashing detergents on enzyme-sensitive soils

Methodology Applied
Scientific EffectSynergistic enzymatic action: Enzyme

Data Source

PatentEP3433351B1Cleaning agent containing protease and amylase
Publication Date: 2026.01.14 HENKEL KGAA
  • EP3433351B1 patent drawing

AI summary

The present invention relates to a cleaning agent, preferably a dishwasher detergent, preferably a hand dishwashing detergent, comprising at least one protease and at least one amylase as defined herein. The invention also relates to the use of said type of cleaning agent for cleaning solid surfaces and to a method for cleaning hard surfaces using the described cleaning agent. The invention further relates to the use of a specific enzyme combination as defined herein for improving the cleaning action of a cleaning agent.