DMP8 DMP9 Proteins Haploid Induction Medicago
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Solution Overview
Problem
Current haploid breeding technologies lack an effective in vivo haploid induction system for legume plants, particularly Medicago truncatula, which hinders the development of new plant varieties and agricultural applications.
Innovation Solution
The development of DMP8 and DMP9 proteins and their encoding genes, along with a CRISPR-Cas9 system, to reduce protein expression and induce haploid plants through gene knockout, facilitating the creation of a haploid induction line in Medicago truncatula using CRISPR-Cas9-mediated gene editing.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If haploid breeding technology is applied to legume plants, then breeding efficiency is improved and breeding period is shortened, but currently no effective in vivo haploid induction system exists for legume plants
Solution Approach 1:
The patent introduces DMP8 and DMP9 proteins as intermediary factors that mediate haploid induction in legume plants. These proteins act as the missing link between existing haploid induction technologies and legume-specific biological systems, enabling the induction process to occur naturally within the plant's cellular environment.
Solution Approach 2:
The patent utilizes CRISPR-Cas9 gene editing to modify specific genetic parameters in Medicago truncatula, creating mutations in DMP8 and DMP9 genes. By changing the genetic state from wild-type to mutant forms, the system enables haploid induction capability that was previously absent in legume plants.
2Productivity
If CRISPR-Cas9-mediated gene knockout is used to reduce protein expression, then haploid induction is achieved, but the complexity of the genetic engineering procedure increases
Solution Approach 1:
The patent divides the complex haploid induction system into discrete, targetable gene components (DMP8 and DMP9). By segmenting the problem into specific gene targets, the CRISPR-Cas9 system can address each component individually through targeted gene knockout, making the overall complex process more manageable and systematic.
Solution Approach 2:
The CRISPR-Cas9 system is designed to autonomously locate and cut the target DNA sequences of DMP8 and DMP9 genes. The system's own guide RNA molecules direct the Cas9 enzyme to the correct genomic locations, enabling self-guided gene knockout without requiring complex external intervention for each editing event.
3Productivity
If DMP8 and DMP9 genes are knocked out using CRISPR-Cas9, then haploid plants can be induced from self-crossed and hybrid offspring, but the precision of gene targeting and editing efficiency must be maintained
Solution Approach 1:
The patent employs a feedback mechanism where guide RNA sequences are specifically designed to match the DMP8 and DMP9 gene sequences. This sequence-specific binding provides feedback control, ensuring that only the intended target genes are cut and modified, thereby maintaining high precision in gene targeting while achieving effective haploid induction.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enables the successful induction of haploid plants from self-crossed and hybrid offspring, enhancing breeding efficiency and shortening the breeding period for leguminous crops like alfalfa, with significant implications for agricultural production.
Implementation Method 1
The development of DMP8 and DMP9 proteins and their encoding genes, along with a CRISPR-Cas9 system, to reduce protein expression and induce haploid plants through gene knockout
Data Source
AI summary
Disclosed in the present invention are a DMP protein and a coding gene and use thereof. Disclosed is a complete set of proteins consisting of protein A (i.e. DMP8) and protein B (i.e. DMP9). The amino acid sequence of protein A is SEQ ID No. 1, and the amino acid sequence of protein B is SEQ ID No. 2. Also disclosed in the present invention are a method for constructing a plant haploid inducer line and a use thereof, and a plant haploid inducer line constructed by the method.