Hydrolases TRIZ Cases

A PPEF-1 gene variant serves as a specific diagnostic marker for detecting T-cell lymphoblastic lymphoma.

Inactivating endogenous lipases in oleaginous yeast boosts retinyl acetate purity to 70-90% while minimizing fatty ester by-products.

Chemically modified artificial nucleic acids recruit endogenous deaminases to target RNA sites, reducing off-target editing caused by hyperactive mutants.

Engineered nitrilases overcome natural enzyme instability by maintaining activity at high substrate concentrations and extreme pH levels.

Novel phospholipase C polypeptide hydrolyzes phospholipids into diacylglycerol and phosphate esters.

Enzymatic hydroxylation replaces complex chemical synthesis to produce cis-5-hydroxy-pipecolic acid efficiently.

A DNA-synthesis recording system maps single-cell connectivity in dense tissues using CRISPR-Cas9 and TdT enzyme activity.

CHANGE-seq applies tagmentation and intramolecular circularization to assess genome editing nucleases, reducing processing time and specialized equipment needs.

A non-naturally occurring RNA molecule forms a complex with an OMNI-103 CRISPR nuclease to target specific DNA sites.

Lipase catalysis replaces harsh alkaline conditions, reducing environmental harm while maintaining production efficiency.

A dual vector system inserts partial ABCA4 coding sequences into host chromosomes using non-homologous end joining.

NHEJ inhibitors suppress non-homologous end joining to boost homologous recombination efficiency, enabling marker-free integration.

Relaxed PAM sequence constraints in modified Cas9 proteins resolve the trade-off between target specificity and cleavage efficiency.

Sulfonate-modified biotin-binding proteins resolve weak molecule retention in sequencing by altering electrostatic parameters.

A DNA end repair reagent combines a combinatorial enzyme with single-strand DNA-binding protein to stabilize sticky ends.

Thermal assembly eliminates machining costs while maintaining zonal isolation precision.

Segmenting guide RNAs enables splint-mediated ligation to resolve synthesis yield bottlenecks and reduce truncation impurities.

Single-chain variable fragment antibodies bind retroviral integrase monomers to prevent dimer formation and block viral DNA integration.

Reducing non-homologous end joining activity via YKU70 integration enables efficient donor DNA insertion and high-titer antibody secretion.

A temperature model estimates solution profiles to calculate creatinine concentrations accurately.

A site-specific nuclease induces genomic breaks repaired via homologous recombination to generate diverse protein variants.

Washing used immobilized lipolytic enzymes with n-hexane reduces residual oil, while alkali treatment desorbs proteins to maintain high DAG purity.

A recombinant enzyme complex fuses PETase and lipase with a mini-scaffolding protein to synergistically break down polyethylene terephthalate.

The Streptococcus thermophilus Csm complex cleaves target RNA at 6-nucleotide intervals using a minimal ribonucleoprotein assembly.

A CRISPR transient expression construct integrates donor polynucleotides via homologous recombination.

CRISPR-Cas9 editing reduces MHC-I expression to evade immune rejection while HLA-E overexpression protects against natural killer cell lysis.

Electroporation delivers nuclease reagents into reproductive organs, bypassing zygote isolation to reduce device complexity while maintaining editing precision.

BE-Hive machine learning model predicts optimal guide RNA and base editor combinations for precise genome editing.

Mutations in the PDX...D/EXK motif and calcium buffers reduce star activity while maintaining precise DNA cleavage.

PTENα-modified stem cells home to tumors, shielding viral vectors from immunity and boosting infectivity.

CRISPR-Cas9 modifies DMP8 and DMP9 genes to enable haploid induction in Medicago truncatula, addressing the lack of effective breeding systems for legumes.

Cas protein-guide RNA ribonucleoproteins edit plant protoplast genomes directly.

Silencing the CGI-58 gene prevents lipid hydrolysis, allowing modified diatoms to store more oil while maintaining robust biomass yield.

A 30S ribosomal protein regulates stomatal aperture to control gas exchange in plant cells.

A split-luciferase reporter system generates luminescent signals upon Cas-cleavage of oligonucleotide linkers attached to color-coded beads.

CRISPR-mediated ZEP gene mutation in Chlamydomonas reinhardtii increases intracellular zeaxanthin concentration.

Adding sodium carbonate buffers metal chelating agents, preserving lipase stability and productivity despite acidic oil environments.

Measuring acid sphingomyelinase enzymatic activity levels in biological samples to detect amyotrophic lateral sclerosis.

Hairpin constraints reduce off-target genome editing while maintaining high modification efficiency.

Combined SOCS1 and PTPN2 guide RNAs resolve unpredictable gene-editing efficacy in T cells.

Morphogenic gene cassettes transform recalcitrant dicot tissues into regenerable structures for transgenic production.

Localized WRKY47 expression in plant roots boosts suberin levels without compromising plant health, enabling effective carbon sequestration.

KRE1 or SWP1 signal peptides enhance co-translational translocation in eukaryotic host cells.

HIV reverse transcriptase enables prime editing with nickase Cas9 to insert genetic sequences.

Sequential chromatography purifies rhDNase I, removing deamidated contaminants to ensure patient safety in cystic fibrosis treatments.

A phytase and alpha amylase enzyme composition liquefies starch at lower temperatures.