DNase Variant Stability in Detergent Compositions
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Solution Overview
Problem
Detergent compositions containing DNase enzymes face challenges with stability in the presence of detergent components like surfactants and bleaches, making them ineffective for removing biofilm soils from hard surfaces and fabrics.
Innovation Solution
A detergent composition incorporating a DNase variant with specific substitutions such as N61D, T65I, T65V, S82R, K107Q, T127S, T127V, G149N, and S164D, which maintains at least 60% sequence identity to a reference DNase, providing improved stability and activity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If a DNase enzyme is used in detergent composition, then biofilm soil removal capability is improved, but stability in the presence of detergent components deteriorates
Solution Approach 1:
The patent applies parameter changes by modifying the amino acid sequence of the DNase enzyme through specific substitutions (N61D, T65I, T65V, S82R, K107Q, T127S, T127V, G149N, S164D, L181S) to alter its physical and chemical properties. These parameter changes in the enzyme's structure enable it to maintain stability in detergent compositions while preserving its biofilm-degrading activity, thus resolving the contradiction between effectiveness and stability.
2Productivity
If DNase enzyme is added to detergent, then stain removal performance is improved, but enzyme activity is lost due to instability
Solution Approach 1:
The patent modifies enzyme parameters through specific amino acid substitutions that enhance the enzyme's resistance to detergent components such as surfactants and bleaches. This allows the DNase to maintain its catalytic activity and stain removal performance throughout the washing process, resolving the contradiction between productivity and reliability.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The DNase variant enhances the stability and wash performance of the detergent, effectively breaking down biofilm soils and improving stain removal on various surfaces, including fabrics and dishware.
Implementation Method 1
deoxyribonuclease (DNase) enzymes... DNase activity... breaking down biofilm soils
Data Source
AI summary
A detergent composition may include a DNase variant and a cleaning adjunct. Also, methods of treating surfaces such as fabrics by contacting the surface with an aqueous wash liquor having the detergent composition therein.