DNase Polypeptides for Laundry Biofilm Removal and Malodor Control

The patent extracts and removes the endogenous inhibitor (PI) from the proteinase preparation through specific purification steps, separating the active enzyme from the inhibitory component. This extraction approach eliminates the need for complex purification procedures while maintaining high proteolytic activity, as the inhibitor is selectively removed rather than dealing with the entire complex mixture.

The patent converts the harmful effect of endogenous inhibitors into a benefit by using them as specific markers for purification. The presence of these inhibitors indicates which fractions contain the active proteinase, allowing selective isolation and concentration of the active enzyme while removing the inhibitors through controlled purification steps, thereby achieving both high activity and stability.

The patent employs a self-service approach where the endogenous inhibitors serve as built-in markers that guide the purification process. By utilizing the inhibitors' presence to identify active enzyme fractions, the system performs its own purification guidance without requiring external complex analytical methods, thereby achieving effective purification with relatively simple and cost-effective procedures.

The patent applies preliminary action by removing endogenous inhibitors before the concentration step through prior purification procedures. By eliminating the aggregating factors (inhibitors) in advance, the subsequent concentration process can proceed without inducing aggregation or activity loss, allowing safe concentration enhancement while maintaining enzyme stability and functionality.