Hydrolases TRIZ Cases

See how engineered host cells with segmented enzymatic pathways replace complex chemical synthe

See how time-separated surfactant and protease dosing improves cleaning performance in high wat

See how engineered cutinase variants with specific amino acid substitutions hydrolyze cyclic ol

See how phenol or o-vanillin swelling agents combined with cutinase enzyme increase polyester h

See how amino acid substitutions at specific positions create protease variants with several hu

See how CE-7 esterase variants lacking the conserved HE submotif enable perhydrolytic peracid p

See how electroblown water-soluble fibers encapsulate enzymes at 25 microns or less to prevent

See how nuclease enzymes with DNase and RNase activity degrade surface nucleic acids to reduce

See how DNase enzyme in detergent breaks down biofilm DNA to reduce fabric creases by up to 90%

Targeted amino acid substitutions in Fe_RF6318 protease improve thermal and detergent stability while preserving low-temperature stain removal.

An acidic Aspergillus phospholipase enables oil degumming at pH 4.0 and 60°C with low lipase activity and residual phosphorus below 10 ppm.

A fungal serine protease maintains stain-degrading activity and detergent stability at low washing temperatures, helping cut energy use.

A fungal serine protease keeps stain-removal activity at low wash temperatures and across varied detergent pH and formulation conditions.

Lipase in polymer coatings breaks down fingerprint oils, while heat speeds volatilization for easy cleaning on high-gloss organic surfaces.

A stable enzyme, peroxide source, and ester composition generates peracetic acid in water on demand, easing storage, transport, and corrosion issues.

Specific NprE mutations prevent citrate-driven calcium loss from triggering autolysis, preserving protease activity in detergent formulations.

Engineered cold-water proteases improve stain removal, whiteness, and freshness in detergent formulations at lower wash temperatures.

Paper spray mass spectrometry detects cholinesterase activity and inhibition in under a minute while also identifying toxicants.

A skin-pricking enzyme patch extracts glycerol from body fat and oxidizes it for power generation with a simpler enzyme system.

Digital data is encoded by the presence or absence of prebuilt nucleic acid sequences, cutting synthesis cost and write time.

Digital bits are stored by the presence or absence of unique nucleic acid sequences, cutting synthesis cost and encoding errors.

Digital data is stored by combining prebuilt unique DNA sequences instead of base-by-base synthesis, cutting encoding cost and complexity.

Pre-synthesized sequence libraries encode bits by presence or absence, avoiding costly base-by-base DNA synthesis and improving retrieval accuracy.

Digital bits are stored through presence-absence pools of unique nucleic acid sequences, avoiding costly base-by-base synthesis.

Digital data is encoded by the presence or absence of prebuilt DNA sequences, avoiding costly base-by-base synthesis for long-term storage.

Whole antibodies are covalently linked to urease with SIAB to improve conjugate stability, binding affinity, and targeted cancer therapy.

A PpCas9 nuclease with a 5′-NNNN(A/G)T-3′ PAM expands targetable DNA sites while preserving specific double-strand cutting.

CRISPR-built PKHD1L1 mutant rats paired with SEP detection provide a standardized model for cortical excitability and anti-epileptic drug testing.

Modular pig gene cassettes regulate coagulation, complement, and innate immunity to reduce rejection and extend xenograft survival.

A pH 3.2-4.5 enzyme treatment suppresses micelle-like behavior in herbaceous biomass extracts, improving solid-liquid separation and clarity.

Continuous or intermittent saccharification enzyme addition keeps fermentation properties in range to sustain ethanol concentration and longer runs.

Specific lipase substitutions at positions 88 and 120 improve L-form selectivity in menthol esterification and hydrolysis, raising optical purity.

A four-gene Neisseria lactamica Type I-C CRISPR complex simplifies mammalian delivery while enabling programmable long-range DNA deletions.

A cleavable X-Y-Z single-stranded nucleic acid enables fast, precise real-time SNP detection in one reaction with less probe complexity.

A plant-optimized Cpf1 and guide polynucleotide complex improves targeting precision, cuts redesign time, and reduces off-target editing in plants.

Point-of-use crRNA generation enables a CRISPR nucleic acid sensor with stable reagents, low-threshold detection, and decentralized testing.

Partial de-glycosylation under native conditions enables rapid glycoprotein conformation comparison using CE-SDS fingerprints.

CRISPR guide RNA and Cas proteins let DNA hydrogels respond to low-concentration nucleic acid triggers without redesigning the matrix.

CRISPR guide RNAs and an endonuclease create female-lethal, sterile male progeny while preserving mating competitiveness for insect control.

Tissue-specific promoters drive recombinase autoexcision to remove marker cassettes in transgenic plants without extra crossing generations.

Guide RNA-directed RGNs improve sequence-specific genome editing by simplifying targeting and reducing off-target effects.

Ptb-Buk reroutes acyl-CoA fermentation to generate ATP, coupling bacterial growth with higher-yield acid, alcohol, and diol production.

A DNase and protease cleaning composition breaks down biofilm and EPS to reduce redeposition, malodor, and persistent stains on surfaces.

Mutating RLK genes in solanaceous plants blocks TSWV infection, spread, and symptoms without harming plant growth.

Site-specific recombinase enables accurate yeast gene integration and marker removal, simplifying transformant verification and reuse.

Protein deamidase tunes egg white heat-coagulated gel strength up or down without the taste changes caused by salt or sugar.

Partially double-stranded 3′ adapters with a cleavable linker cut adapter dimers and ligation bias in small RNA sequencing libraries.

Proximity-driven nucleic acid assembly activates CRISPR-Cas reporters for sensitive analyte detection in low-cost point-of-care assays.

Single crRNA Cpf1 editing expands access to AT-rich DNA while improving mammalian genome editing efficiency and RNA simplicity.

A continuous low-porosity coating enables higher enzyme payloads while limiting activity loss during steam pelleting.

DNase-active polypeptides break down biofilm DNA in laundry, improving soil removal, reducing malodor, and preserving fabric whiteness.

Enzymatic hydrolysis turns Gryllus bimaculatus into a water-soluble extract rich in polypeptides and free amino acids for human food use.

Novel HAD-like terpene synthases convert FPP into drimenol and albicanol with high selectivity, reducing synthesis complexity and cost.

A two-component guide RNA and Cas9 approach enables site-specific eukaryotic genome editing without bacterial RNA processing machinery.

Five optimized Egt genes enable rice seeds to biosynthesize ergothioneine with easier extraction, higher purity, and lower cost.

Breakpoint-specific dCas9 and gRNA labeling makes ecDNA visible in cancer cells without cleavage, aiding tracking of resistance and recurrence.

Adding transcription factor binding sites to donor DNA improves nuclear import and shifts repair toward HDR for more precise genome insertion.

Programmable Cas13 orthologs use guide RNAs to target eukaryotic transcripts with high specificity for knockdown, binding, imaging, and editing.

Promoter trapping and short homology arms improve HDR in mammalian genome editing, enabling faster large DNA integration without clonal isolation.

Targeted amino acid substitutions improve carboxyesterase hydrolysis, stability, and chiral resolution under industrial conditions.

Mutating AsCpf1 residues R951 and R955 weakens nonspecific DNA hydrogen bonding, cutting off-target editing while preserving cleavage activity.

Dispersed genome repeat sites are cleaved with CRISPR constructs to disable flanking genes and curb pathogen resistance development.

Hepatocyte-targeted CRISPR/AAV editing of the AGT gene enables sustained blood pressure reduction after a single administration.

Photocaged guide RNAs keep Cas9 pre-bound but inactive until light removes the roadblock, enabling second-scale, submicron DNA cleavage control.

Targeted FnCpf1 mutations expand PAM recognition beyond T-rich sites, improving GC-rich genome editing while retaining TTTV activity.

Phage-derived particles package and deliver replicative DNA vectors into C. acnes in situ, enabling broader strain modification across skin microbiomes.

A cold-active VSW-3 RNA polymerase enables precise short RNA synthesis without dsRNA contamination, 3′ extensions, or Class II termination.

Multi-mode chromatography plus caprylate precipitation removes HCP and impurities while preserving heparan-N-sulfatase stability.

Flap cleavage and adaptor ligation remove PAM limits in CRISPR nucleic acid detection, enabling faster and more specific SNP readouts.

CRISPR/Cas9 knockout of TaCIPK14 alleles boosts wheat resistance to Fusarium head blight while preserving normal agronomic traits.

Engineered bacteria combine cyanide lixiviant production, metal reduction, and cyanolysis to recover e-waste metals while detoxifying cyanide.

Single-site PHB depolymerase mutations improve solubility and stability for high-salt, high-temperature PHA degradation and decontamination.

Mutated Cpf1 enzymes alter PAM recognition to reach more genomic sites while preserving precise guide-directed targeting.

Stacked gelatin layers and CRISPR/Cas13a chambers enable non-invasive RNA disease risk assessment and detection from minimal biological fluid.

pH-responsive nanoparticle aggregates enable reversible colorimetric protease detection in undiluted biological fluids with fewer false positives.

Suppressing salicylic-acid-linked plant defenses enables stronger exogenous gene expression and higher target protein production.

Enzyme cascades using enal-cleaving polypeptides and BVMOs shorten terpene chains stepwise to make defined perfumery intermediates.

Monoclonal antibodies and a biotin-streptavidin ELISA setup enable specific, repeatable PADI4 detection in tumor serum.

An integration-deficient lentiviral vector pairs with serine recombinase to avoid random genome insertion while enabling precise gene targeting.

PARP2 disruption shifts tomato biomass from stems and leaves to fruit, enabling compact plants with faster harvests in confined growing spaces.

CRISPR/Cas-ready tau biosensor cells enable screening of genes that enhance or inhibit tau seeding and aggregation in neurodegenerative disease research.

CRISPR editing of endogenous cytokinin receptor HK genes fine-tunes hormone pathways to improve seed size and oil content without transgenes.

Uses endonuclease cleavage plus homologous recombination to truncate and join DNA fragments at scale with fewer enzymes and purification steps.

Biolistic delivery of nuclease proteins or RNPs into dry embryo explants avoids callus culture and broadens plant genome editing across germplasms.

Serum LTA4H and METRNL ratio scoring standardizes PCOS diagnosis, improving consistency and earlier assessment in young women.

A genetic circuit modulates homeobox and transcription factor expression to convert stem cells into chondrogenic cells faster within 7 days.

Targeted YhhS amino acid substitutions boost O-phosphoserine export, raising OPS yield and improving cysteine production efficiency.

Sequential sodium chlorite and hydrogen peroxide decolorization lowers PHA b* value below 15 while preserving molecular weight and purity.

CRISPR guide RNA and Cas target cancer-specific sequences to insert a suicide gene, killing cancer cells while sparing healthy cells.

Targeting RAP2C expression improves placental angiogenesis and nutrient transport to support higher calf birth weight in Blcattle black cattle.

Engineered plasmid templates and ligation steps raise circRNA yield while avoiding immune-activating double-stranded stem structures.

Small CD73-targeting nanobody-drug conjugates improve solid tumor penetration and drug delivery while blocking CD73 activity and limiting normal-tissue exposure.

CRISPR-edited LRR-RLK alleles expand maize meristems to raise kernel row number while preserving organized growth and ear length.

FcRγ-deficient CAR-NK cells paired with a monoclonal antibody boost dual-antigen recognition and cytolytic killing in cancer treatment.

Engineered plasma cells use HDR-based gene insertion to secrete BTCEs long term, avoiding repeated high-dose infusions and improving cancer cell killing.

Specific lipase substitutions shift activity away from neutral pH to cut rinse-cycle odor while preserving alkaline wash cleaning and storage stability.

Magnetic microparticles and inactivated genetic scissors speed nucleic acid detection while reducing false positives for point-of-care diagnosis.

Guide oligonucleotides recruit endogenous ADAR to edit NRF2 or KEAP1 RNA, improving disruption specificity and therapeutic stability.

Chemical nucleotide substitutions in crRNA and tracrRNA tune Cas9 cleavage kinetics, stability, and specificity while reducing off-target editing.

RNA-triggered cgRNAs switch Cas activity on or off through allosteric conformational change, enabling cell-selective CRISPR regulation.