Doubly Labeled Water Protocol for Uncooled Urine Shipment
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Solution Overview
Problem
Existing doubly labeled water (DLW) analysis techniques are limited by the need for controlled conditions and supervised sample collection, making it difficult for laboratories to provide remote analysis services efficiently and cost-effectively.
Innovation Solution
A method involving reduced isotope dosages of deuterium and oxygen-18, uncooled sample transportation, and parallel measurement techniques using liquid water isotope analyzers to determine metabolic parameters, enabling remote and cost-effective DLW analysis.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If traditional DLW analysis with higher isotope dosages and cooled sample storage is used, then measurement accuracy is maintained, but cost and complexity increase
Solution Approach 1:
The patent changes the parameters of isotope dosage (reducing deuterium to <0.12 g/kg and oxygen-18 to <0.18 g/kg) and sample storage temperature (from cooled to uncooled), demonstrating that accurate metabolic measurements can be achieved with modified parameters that reduce system complexity and cost
Solution Approach 2:
The patent uses slightly lower isotope dosages than traditional methods, which is sufficient to achieve accurate measurements without requiring excessive isotope amounts, thereby reducing cost and simplifying sample handling requirements
2Reliability
If traditional DLW analysis with supervised sample collection is used, then measurement reliability is ensured, but service accessibility and productivity decrease
Solution Approach 1:
The patent enables participants to collect and ship samples independently without supervisor presence, with the robust protocol ensuring reliable measurements are obtained through self-directed sample collection and uncooled transportation to the laboratory
3Measurement precision
If traditional DLW analysis with higher isotope dosages is used, then measurement accuracy is maintained, but cost increases
Solution Approach 1:
The patent optimizes the isotope dosage parameters by reducing deuterium to less than 0.12 g/kg and oxygen-18 to less than 0.18 g/kg, demonstrating that accurate metabolic parameter measurements can be achieved with lower isotope amounts through the enhanced analytical protocol
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach allows for accurate metabolic parameter determination with reduced isotope usage and transportation costs, improving efficiency and reducing turnaround time while maintaining high measurement accuracy.
Implementation Method 1
An isotope analyzer processes the urine samples to determine the rate at which the person's body eliminated deuterium and oxygen-18
Data Source
AI summary
Doubly labeled water with enhanced protocol. One embodiment is a method including providing, to a user, a doubly labeled water (DLW) dose for the user to ingest, the DLW dose including deuterium and oxygen-18, wherein an amount of the deuterium is less than 0.12 grams per kilogram (g/kg) of body water of the user, and wherein an amount of the oxygen-18 is less than 0.18 g/kg of body water of the user. The method also includes receiving, from the user, a non-cooled shipment of urine samples collected in connection with ingestion of the DLW dose, wherein the urine samples remain uncooled after collection and during transit for a period of up to 24 days. The method further includes processing the urine samples with a liquid water isotope analyzer to determine one or more metabolic parameters of the user.