Differential Scanning Calorimetry for Target Molecule Detection in Complex Fluids
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Solution Overview
Problem
Current methods for detecting and analyzing target molecules in complex fluids, such as biologics and viruses, are hindered by the need for costly and time-consuming purification processes, which can alter the structure and binding characteristics of proteins, leading to inaccurate results and increased development costs.
Innovation Solution
A system and method utilizing differential scanning calorimetry (DSC) to analyze complex fluids directly, comparing thermograms of samples with and without target molecules, allowing for the identification and quantification of target molecules without prior purification, and assessing ligand binding and protein interactions in their native state.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If purification processes are used to detect and analyze target molecules in complex fluids, then measurement precision is improved, but loss of time and loss of substance increase significantly
Solution Approach 1:
The patent extracts the target molecule's thermal signature from the complex fluid background by using differential scanning calorimetry to measure heat capacity changes specifically attributable to the target molecule, separating the signal of interest from the complex matrix without physical purification
Solution Approach 2:
The patent uses thermal energy as an intermediary to detect target molecules indirectly through their heat capacity effects, allowing detection without direct physical separation or purification of the target from the complex fluid
2Measurement precision
If purification processes are used to detect and analyze target molecules in complex fluids, then measurement precision is improved, but manufacturing cost increases
Solution Approach 1:
The method allows the complex fluid to serve itself as the analysis matrix, eliminating the need for external purification services or processes. The target molecule's thermal properties enable direct detection in the native complex fluid environment
Solution Approach 2:
The patent replaces mechanical purification systems with a thermal analysis approach, substituting physical separation processes with calorimetric measurement that detects target molecules through their thermal signatures in the complex fluid
3Measurement precision
If purification processes are used to detect and analyze target molecules in complex fluids, then measurement precision is improved, but the target molecule's structure and binding characteristics are altered
Solution Approach 1:
The patent extracts only the thermal information specific to the target molecule from the complex fluid, obtaining the detection signal without physically removing or isolating the target molecule, thereby preserving its native structure and interactions
Solution Approach 2:
The patent uses thermal energy as an intermediary to probe the target molecule's structure and binding characteristics indirectly, allowing measurement of structural properties without physical manipulation that could alter the molecule's native state
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables rapid and accurate detection and analysis of target molecules and ligand interactions in complex fluids, reducing development costs and time by avoiding the need for purification, while providing insights into structural integrity and binding efficacy.
Implementation Method 1
obtain an analysis sample thermogram by differential scanning calorimetry
Data Source
AI summary
Methods for detecting, identifying, and/or quantifying a target molecule in a complex fluid using thermal analysis are disclosed. Exemplary complex fluids include biofluids and environmental fluids. Exemplary target molecules include proteins, peptides, nucleic acids, lipids, carbohydrates, viruses, and combinations thereof. A method for using thermal analysis to determine whether purification affects one or more characteristics, such as binding characteristics, of a target molecule is also disclosed.