Multiplexed Analyte Characterization Using Dye-Tagged Affinity Molecules
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Solution Overview
Problem
Current analytical methods for characterizing target analytes in the pharmaceutical, chemical, and life sciences industries often require multiple assays to collect data on multiple attributes, which is burdensome in terms of sample preparation, reagent costs, and time.
Innovation Solution
The use of dye-tagged affinity molecules (DTAMs) in liquid chromatography systems, where DTAMs are added to samples, injected into a liquid chromatographic system with detectors, and multiple attributes of target analytes are measured simultaneously.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If multiple distinct assays are used to collect data on multiple attributes, then measurement precision is improved, but loss of time and device complexity increase
Solution Approach 1:
The patent combines multiple detection functions into a single liquid chromatography system by integrating multiple detectors (e.g., UV-Vis, fluorescence, mass spectrometry, light scattering) that can simultaneously measure different attributes of analytes in one assay, eliminating the need for separate assays for each attribute
Solution Approach 2:
The liquid chromatography system is designed with multi-functionality to perform various characterization tasks (size, mass, charge, quantity) using different detector combinations, making a single platform capable of replacing multiple specialized assays
2Measurement precision
If multiple distinct assays are used to collect data on multiple attributes, then measurement precision is improved, but reagent expenses and sample preparation burden increase
Solution Approach 1:
Multiple detection functions are merged into a single assay platform, allowing simultaneous measurement of multiple attributes using one set of reagents and sample preparation protocol, thereby reducing reagent consumption and sample preparation burden compared to running separate assays for each attribute
3Measurement precision
If separate column/detector combinations are used for different analyses, then measurement precision is improved, but device complexity increases
Solution Approach 1:
The liquid chromatography system is designed as a universal platform that can accommodate various column types and detector combinations for different analyses, providing a single integrated system that replaces multiple separate configurations while maintaining measurement precision for different attributes
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method allows for the simultaneous quantification and characterization of multiple attributes of target analytes in a single assay, reducing the need for multiple assays and improving efficiency and cost-effectiveness.
Implementation Method 1
D represents an ultraviolet (UV) or fluorescent dye molecule, -L-represents an optionally substituted aliphatic, aromatic, heterocyclic, polyethylene glycol, carbohydrate, or peptide linker, and M is an affinity group
Implementation Method 2
injecting the sample into a liquid chromatographic system comprising at least one detector
Implementation Method 3
D represents an ultraviolet (UV) or fluorescent dye molecule
Implementation Method 4
D represents an ultraviolet (UV) or fluorescent dye molecule
Data Source
AI summary
Disclosed herein are methods for the multiplexed characterization of analytes using dye-tagged affinity molecules. The DTAMs, useful in conjunction with liquid chromatography systems, allow for the parallel quantitation and characterization of attributes of one or more target analytes from complex solutions.