Electrode Array Multi-Analyte Detection Using AC Dielectrophoresis
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Solution Overview
Problem
Current biological assays often require separate analysis of different types of macromolecules from complex samples, leading to inefficiencies and biases, and lack the capability to simultaneously detect multiple analytes with high accuracy.
Innovation Solution
The use of an electrode array configured to generate AC dielectrophoretic fields to capture and detect multiple analytes, including DNA, RNA, nucleosomes, exosomes, and proteins, by creating low and high field regions to isolate and quantify these analytes simultaneously.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If separate analysis of different types of macromolecules is performed, then each analyte can be analyzed with dedicated methods, but the overall analysis process becomes complex and time-consuming
Solution Approach 1:
The patent applies universality by developing a single electrode array system that can detect multiple types of macromolecules (DNA, RNA, proteins, exosomes) simultaneously using the same platform and reagents, eliminating the need for separate dedicated assays for each analyte type while maintaining detection accuracy
Solution Approach 2:
The patent merges multiple separate analysis workflows into a single integrated assay by combining capture probes for different analyte types on the same electrode array, allowing simultaneous detection of multiple macromolecule types in one experiment rather than requiring separate analyses
2Measurement precision
If separate analysis of different types of macromolecules is performed, then each analyte can be analyzed independently, but the overall analysis time increases
Solution Approach 1:
The patent enables continuous detection of multiple analyte types simultaneously on the same electrode array without interruption, where all analytes are captured and detected in parallel during a single assay run, eliminating the sequential time loss inherent in separate analyses
Solution Approach 2:
The patent combines multiple detection workflows into a single parallel process, where DNA, RNA, proteins, and exosomes are all captured and detected simultaneously on the same platform, reducing total analysis time from multiple sequential steps to one integrated assay
3Measurement precision
If isolation of target molecules is performed before analysis, then detection accuracy can be improved, but biases are introduced and workflow complexity increases
Solution Approach 1:
The patent performs preliminary capture of all target macromolecules directly on the electrode array surface using specific capture probes, concentrating analytes at the detection site without requiring bulk sample isolation steps that could introduce bias or loss
Solution Approach 2:
The patent extracts only the essential capture and detection functions to the electrode array surface, eliminating the need for complex bulk sample isolation and purification steps that can introduce biases, while maintaining detection accuracy through localized probe-based capture
4Measurement precision
If multiple analytes are detected simultaneously, then assay accuracy and confidence increase, but the device complexity increases
Solution Approach 1:
The patent achieves multi-analyte detection using a universal electrode array platform that handles DNA, RNA, proteins, and exosomes with the same basic detection mechanism and reagents, avoiding the need for multiple specialized detection systems
Solution Approach 2:
The patent applies local quality by placing specific capture probes at specific locations on the electrode array surface, where each probe type is localized to its functional position, allowing multiple analyte types to be detected simultaneously through spatially organized but otherwise simple probe-based capture
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach increases the accuracy and precision of disease detection and diagnosis by up to 1000% compared to single-analyte methods, with reduced false positives and negatives, and simplifies workflows by allowing simultaneous detection and quantification of multiple analytes.
Implementation Method 1
capturing a plurality of analytes in the biological sample using an electrode configured to generate an AC dielectrophoretic field
Data Source
AI summary
The present invention includes methods, devices and systems for isolating, identifying, analyzing, and quantifying biological materials from fluid samples. In various aspects, the methods, devices and systems may allow for a rapid procedure that requires a minimal amount of material and/or results in high purity biological materials from complex fluids such as blood, serum, or plasma.