Enzymatic Esterification of Butyric Acid Using Lipase

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Solution Overview

Problem

Conventional synthesis processes for glyceryl butyrate require chemical catalysts and water-carrying agents, leading to high energy consumption and low catalytic efficiency due to the unique physical and chemical properties of butyric acid, which results in low conversion rates during esterification reactions.

Innovation Solution

An enzymatic method using lipase as a catalyst with ethyl acetate or ethyl formate as additives for esterification between n-butyric acid and glycerol at normal temperature and pressure without a water-carrying agent, optimizing the reaction conditions to enhance catalytic efficiency.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If conventional chemical catalysts and water-carrying agents are used for esterification, then the reaction can proceed, but energy consumption increases and catalytic efficiency decreases

Engineering Contradiction:
Improveconversion rate of butyric acidVSAvoidenergy consumption
Core Design Contradiction:
ProductivityVSUse of energy by moving object

Solution Approach 1:

The invention changes the reaction parameters by using lipase enzyme catalyst instead of conventional chemical catalysts, conducting the reaction at normal temperature and pressure without water-carrying agents. This enzymatic approach achieves high conversion rates of butyric acid while significantly reducing energy consumption compared to traditional high-temperature chemical catalysis methods

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The invention introduces ethyl acetate or ethyl formate as reaction assistants that act as intermediaries to enhance the esterification reaction. These additives improve the catalytic efficiency of lipase and facilitate the reaction between butyric acid and glycerol, achieving high conversion rates without requiring energy-intensive water-carrying agents

Inventive Principle:
Principle #24Intermediary (Mediator)

2Productivity

If conventional chemical catalysts are used, then esterification reaction can occur, but the process becomes complicated and toxic water-carrying agents are required

Engineering Contradiction:
Improveconversion rate of butyric acidVSAvoidtoxic water-carrying agents
Core Design Contradiction:
ProductivityVSObject-generated harmful factors

Solution Approach 1:

The invention replaces toxic water-carrying agents with biodegradable ethyl acetate or ethyl formate as reaction assistants. These additives are environmentally friendly, non-toxic, and can be easily removed from the final product, eliminating the harmful effects associated with conventional water-carrying agents while maintaining high conversion rates

Inventive Principle:
Principle #27Cheap short-living objects (Disposable)

Solution Approach 2:

The invention uses ethyl acetate or ethyl formate as safe intermediary substances that facilitate the esterification reaction without introducing toxicity. These reaction assistants effectively replace harmful water-carrying agents, enabling the reaction to proceed with high efficiency while producing no harmful waste

Inventive Principle:
Principle #24Intermediary (Mediator)

3Speed

If conventional high-temperature esterification is used, then reaction speed increases, but energy consumption increases and catalytic efficiency decreases

Engineering Contradiction:
Improvereaction speedVSAvoidenergy consumption
Core Design Contradiction:
SpeedVSUse of energy by moving object

Solution Approach 1:

The invention replaces the mechanical/thermal energy-driven chemical catalysis system with a bio-enzymatic system. Lipase enzyme catalyst enables the esterification reaction to proceed rapidly at normal temperature, substituting the need for high-temperature heating and achieving both fast reaction speed and low energy consumption simultaneously

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

This method reduces energy consumption and increases the conversion rate of butyric acid to glyceryl butyrate, achieving higher yields and avoiding the use of toxic water-carrying agents, while maintaining a safe and non-toxic process.

Implementation Method 1

carrying out an esterification reaction between n-butyric acid and glycerol using lipase as a catalyst

Methodology Applied
Scientific EffectEnzymatic catalysis: Enzyme

Implementation Method 2

esterification reaction between n-butyric acid and glycerol

Methodology Applied
Scientific EffectEsterification: Chemical Bonding

Implementation Method 3

ethyl acetate and/or ethyl formate as an additive

Methodology Applied
Scientific EffectSolvation: Solvation

Implementation Method 4

separating the reaction product, recovering the additive by distillation under reduced pressure

Methodology Applied
Scientific EffectDistillation: Distillation

Implementation Method 5

distillation under reduced pressure

Methodology Applied
Scientific EffectVacuum distillation: Vacuum Distillation

Data Source

PatentUS10870869B2Enzymatic method for preparing glyceryl butyrate
Publication Date: 2020.12.22 SOUTH CHINA UNIV OF TECH

AI summary

An enzymatic method for preparing glyceryl butyrate, comprising: (1) carrying out an esterification reaction between n-butyric acid and glycerol using lipase as a catalyst, ethyl acetate and/or ethyl formate as an additive; (2) separating the reaction product, recovering the additive by distillation under reduced pressure to obtain glyceryl butyrate. The invention uses lipase as a catalyst to catalyze the esterification reaction of n-butyric acid and glycerin at normal temperature and normal pressure, reduces the energy consumption of the reaction. Also, the reaction condition is mild, no side reaction happens, and no water-carrying agent is used. In addition, the additive increases the catalytic efficiency of the lipase and the conversion rate of butyric acid.