Hydrolases TRIZ Cases

Targeted gene mutations in soybean plants increase saturated fatty acids, avoiding trans fats from chemical hydrogenation and palm oil sustainability issues.

Low percentage ECM suspension culture supports large-scale organoid expansion, reducing costs and labor intensity for high-throughput screening.

Domain swapping in variant acyl-ACP thioesterases improves fatty acid profile control while managing enzyme structural complexity.

Serum ACY-1 measurement overcomes urinary marker confounding by urine production issues to accurately predict delayed graft function.

Chimeric transposases fuse DNA-binding domains to enhance integration efficiency while reducing mutagenesis risk.

Tobacco rattle virus delivers guide RNA and Cas9 endonuclease to bypass stable transformation time.

AceCas9 utilizes a cytosine-specific Cas9 endonuclease to target 5'-NNNCC-3' PAM sequences for precise genome editing.

Cas12b nuclease introduces targeted double-stranded breaks in plant genomic DNA, resolving random modification precision issues.

Feruloyl esterase removes toxic esters from pretreated cellulosic liquor, resolving inhibition of cellulase enzymes and improving saccharification yield.

Engineered CytoRP targets aminoacylatable 3′ tRNA-like structures, reducing viral replication and crop yield losses.

Endonuclease Q targets the 5' side of damaged bases to resolve gaps requiring additional lyase activity.

Segmented expression systems using different promoters overcome low cutting rates by providing multiple guide RNAs simultaneously.

RNA-guided nuclease targets mutant CXCR4 alleles via guide RNA sequence recognition, preserving functional protein expression.

CRISPR-Cas9 targets upstream open reading frames to resolve translation inhibition and control protein levels.

Inhibiting p53 pathways reduces nuclease toxicity, increasing gene editing efficiency in stem cells.

A targeted gene editing system guides non-nuclease effector proteins to specific DNA sequences using a sequence-targeting protein and RNA scaffold.

Full-length RecE exonuclease paired with RecT annealing protein drives efficient homologous recombination between nucleic acid molecules.

Dynamic PepC protease regulation via a controlled promoter resolves the contradiction between high polypeptide yield and impaired sporulation ability.

Engineered microbial cells remove specific enzymes to reduce byproduct formation and improve product purity in biosynthetic pathways.

Atomic structures of Cas9 polypeptides resolve structural analysis complexity by revealing guide RNA recognition mechanisms for precise genome editing.

Site-directed mutagenesis of fructosyl amino acid oxidase resolves kit instability caused by poor enzyme thermostability.

Hybridization generates enzyme cleavage sites for temporally controlled effector removal, addressing the lack of temporal control in genetic approaches.

Guide RNA and Cas endonuclease target MS9, MS22, MS26, or MS45 genes to eliminate random mutagenesis screening.

Engineering RuvC and HEPN domains resolves the trade-off between targeting specificity and cleavage efficiency in genome editing applications.

Enhancing acyl-CoA synthetase and thioesterase expression in Nannochloropsis transformants to boost lipid yields.

Intermediate fusion chromosomes with selectable markers resolve precision and efficiency trade-offs in chromosome rearrangement.

Magnetic spheres coated with anti-Lp-PLA2 antibodies enable automated chemiluminescence immunoassay detection.

A programmable DNA binding unit directs a transposome to specific genomic sites for targeted fragmentation and sequencing library generation.

Electroporation delivers Cas9 ribonucleoproteins to primary hematopoietic cells, overcoming low efficiency limits in stem cell genome editing.

Targeted editing of the CTLA4 gene intron restores regulated expression while avoiding supraphysiological levels and insertional mutagenesis risks.

Library of constitutive and inducible promoters enables precise gene expression control in Bacteroides species.

Removing glutamate breakdown pathways directs carbon flux toward product formation, increasing yield and purity without complex purification.

Specific amino acid substitutions at positions 82 and 84 improve hydrogen peroxide stability, eliminating the need for external stabilizing agents.

A chip-based biosensor system measures biomolecular binding interactions to determine genome manipulating efficiency parameters.

A plasmid backbone with type IIs restriction sites enables modular assembly of guide RNA and transfer RNA sequences for multiplexed genome editing.

Amino acid substitutions enhance variant phytase thermostability, preventing enzyme inactivation during feed pelleting and improving nutrient availability.

Mutated phospholipase C hydrolyzes phosphatidylcholine, reducing energy consumption and material losses in oil degumming.

Tethering a functional RNA to Cas12a crRNA through a 3' extension boosts genome editing efficiency without requiring separate tracrRNA components.

Modified protease variants withstand bleach and complexing agents to remove proteinaceous soils in soft water.

Recombinant DNA constructs deliver guide polynucleotides and endonucleases to organelles for targeted genome alteration.

L6 gene confers broad-spectrum resistance to Peronospora farinosa in spinach plants.

Substituting threonine for lysine at position 231 of the thioesterase sequence boosts fatty acid productivity beyond wild-type enzyme levels.

A restriction endonuclease targets the 5'-TCGA-3' sequence to detect cytosine methylation status in genomic regions.

A P.vNuc enzyme degrades nucleic acids to reduce viscosity in cell culture solutions.

Mutated PYR/PYL receptor proteins increase abscisic acid sensitivity through targeted amino acid substitutions at the PP2C-binding interface.

TET enzymes and glycosyltransferases distinguish low-abundance cytosine derivatives, resolving identification precision limits in epigenetic analysis.

Knocking out TCR and HLA-A genes in universal CAR-T cells reduces immune rejection while improving amplification efficiency for neuroblastoma treatment.

Lipase catalyzes esterification between n-butyric acid and glycerol, reducing energy consumption by eliminating high-temperature heating.