Protease Variant for Dishwashing Detergent Stability
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Solution Overview
Problem
Automatic dishwashing compositions face challenges in effectively removing proteinaceous soils, particularly in soft water and under stressed conditions such as high temperatures and long cycles, due to the negative impact of complexing agents and bleach on protease stability and performance.
Innovation Solution
A phosphate-free automatic dishwashing detergent composition incorporating a specific variant protease with enhanced stability and a complexing agent system, along with a high bleaching system, designed to maintain performance in soft water and withstand harsh conditions.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Object-affected harmful factors
If complexing agents are used in high levels to replace phosphate, then environmental profile is improved, but protease stability is negatively affected
Solution Approach 1:
The patent modifies the protease enzyme sequence through specific amino acid substitutions (e.g., at positions 195, 205, 211, 212, 253, 256) to change its structural and functional parameters. This allows the protease to maintain stability and activity despite the presence of complexing agents, resolving the contradiction between environmental improvements and enzyme reliability.
Solution Approach 2:
The invention creates a composite system combining the modified protease with specific stabilizing agents and complexing agents in a detergent formulation. This composite approach allows the protease to function effectively while maintaining stability in the presence of high levels of complexing agents, achieving both environmental and performance goals.
2Productivity
If high level of bleach is added to provide good cleaning, then cleaning performance is improved, but protease performance is impaired
Solution Approach 1:
The patent introduces specific amino acid changes in the protease sequence that modify its structural parameters to reduce sensitivity to oxidative stress from bleach. These parameter changes enable the protease to maintain activity and stability even in formulations with high bleach concentrations, allowing both strong cleaning performance and enzyme reliability.
3Productivity
If high temperature and long cycles are used for heavily soiled loads, then cleaning effectiveness is improved, but protease stability is reduced
Solution Approach 1:
The invention introduces specific amino acid substitutions that alter the protease's thermal stability parameters. These changes enable the enzyme to maintain its structural integrity and catalytic activity at elevated temperatures and over extended periods, allowing effective cleaning of heavily soiled loads while preserving protease stability under stressed conditions.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The composition achieves improved stability and cleaning performance on proteinaceous soils like egg and crème brûlée, even in soft water and under hot, long cycles, ensuring effective removal and stability of the protease.
Implementation Method 1
The composition presents improved stability and/or performance on egg and/or crème brulee removal
Implementation Method 2
complexing agents can have a strong binding capacity for metals and/or are used in high levels and can negatively affect the stability of enzymes, in particular complexing agents can negatively affect proteases by extracting the structural calcium metal ions of the protease
Implementation Method 3
While compositions having a high level of bleach can provide good cleaning the bleach can also impair on the performance of enzymes, specifically proteases
Data Source
AI summary
An automatic dishwashing cleaning composition having a new protease.