Epicardial SVF Construct for Myocardial Infarction Treatment
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Solution Overview
Problem
Current cell-based therapies for treating myocardial infarction face challenges such as limited retention of cells in the target tissue and inadequate angiogenesis, with existing delivery methods like intramyocardial injections resulting in low cell survival and engraftment.
Innovation Solution
A stromal vascular fraction (SVF) construct is applied to the epicardial surface of the heart, seeded onto a biocompatible substrate like VICRYL® brand polyglactin 910 mesh, to enhance cell retention and promote neovascularization at the site of myocardial infarction.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of operation
If intramyocardial injections are used to deliver cells, then cell delivery is simple, but cell retention and engraftment are low (only 1.3-2.6% detected in infarcted myocardium within 50-75 minutes)
Solution Approach 1:
An extracellular matrix (ECM) hydrogel is introduced as an intermediary carrier between the cells and the myocardial tissue. The hydrogel serves as a temporary scaffold that retains cells at the injection site and facilitates their gradual integration into the host tissue, solving the problem of poor cell retention associated with direct intramyocardial injection
Solution Approach 2:
The ECM hydrogel is pre-formed and prepared before cell injection, creating a ready-made three-dimensional structure that is specifically designed to capture and retain cells. This preliminary preparation ensures that cells are immediately captured upon injection, preventing their rapid washout from the target tissue
2Quantity of substance
If cell-based therapy is applied to promote angiogenesis, then perfused vessels increase, but the extent of angiogenesis remains inadequate
Solution Approach 1:
The ECM hydrogel is specifically designed with localized properties that promote angiogenesis, including incorporated growth factors and a three-dimensional structure that mimics native extracellular matrix. This creates a favorable local microenvironment that enhances the efficiency of angiogenesis directly at the infarcted myocardium site
Solution Approach 2:
The ECM hydrogel combines multiple components including extracellular matrix proteins, growth factors, and scaffolding materials to create a composite structure that simultaneously provides mechanical support, cell retention, and angiogenic stimulation, thereby enhancing overall therapy effectiveness
Data Source
AI summary
This application discloses methods for treating an established myocardial infarction, including treatment with an epicardial construct containing stromal vascular fraction (SVF) from adipose tissue which may be seeded onto a biocompatible substrate, which preserves microvascular function and LV contractile mechanisms.