Extracellular Vesicle Targeting via Controlled Shockwave Energy
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Solution Overview
Problem
Existing methods for delivering target materials into extracellular vesicles, such as exosomes, are inefficient and often damage cells when using high-energy extracorporeal shockwaves, limiting their application in drug delivery and therapy.
Innovation Solution
Exposing target materials and extracellular vesicles to a controlled range of extracorporeal shockwaves with energy densities between 0.05 mJ/mm² and 0.9 mJ/mm², specifically 0.05 mJ/mm² to 0.70 mJ/mm², to enhance the incorporation of target materials into vesicles without cell damage, using exosomes derived from natural or artificial sources.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If high-energy extracorporeal shockwaves are used to deliver target materials into extracellular vesicles, then the delivery efficiency is improved, but cell damage occurs
Solution Approach 1:
The patent applies parameter changes by precisely controlling the energy density of extracorporeal shockwaves within the range of 0.05-0.9 mJ/mm². This optimization of physical parameters enables effective target material delivery into extracellular vesicles while preventing cell damage that occurs with higher energy levels. The specific energy range represents a critical parameter adjustment that resolves the contradiction between delivery efficiency and cell safety.
2Reliability
If conventional methods are used to deliver target materials into extracellular vesicles, then cell safety is maintained, but delivery efficiency is low
Solution Approach 1:
The patent employs periodic action through the application of pulsed extracorporeal shockwaves. The shockwaves are delivered in controlled pulses with specific energy densities (0.05-0.9 mJ/mm²), creating periodic mechanical stress that facilitates target material entry into extracellular vesicles. This periodic mechanical stimulation achieves high delivery efficiency while the controlled pulse parameters ensure cell safety, resolving the contradiction between reliability and productivity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method significantly increases the efficiency of target material delivery into cells while maintaining the stability and immune-rejection-free nature of extracellular vesicles, expanding their use as drug delivery vehicles across various fields.
Implementation Method 1
exposing the target material and the extracellular vesicle to an extracorporeal shockwave
Data Source
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AI summary
The present disclosure relates to a method of delivering target materials into extracellular vesicles including exposing the target materials and the extracellular vesicles to extracorporeal shockwaves, a method of preparing target material-introduced extracellular vesicles, extracellular vesicles prepared by the method, drug delivery vehicles including extracellular vesicles, and a method of delivering target materials into cells. The present disclosure exposes extracellular vesicles derived from natural organisms such as animal cells, plant cells, and microorganisms including bacteria and eukaryotic bacteria as well as artificially produced extracellular vesicles to extracorporeal shockwaves extracellularly. Thus, the high-level energy extracorporeal shockwaves can be used to deliver the target material into the extracellular vesicle efficiently. When treating with extracorporeal shockwaves, the ability of target material-introduced extracellular vesicles to incorporate into target cells also increases. According to the method of the present disclosure, the target materials can be delivered into cells with high efficiency, being utilized in various fields.