Feline Probiotic Lactobacilli Isolation from Upper GI Tract
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Solution Overview
Problem
There is a need for probiotic strains of lactic acid bacteria specifically adapted for cats, derived from their natural intestinal microflora, that can survive processing and maintain health benefits for companion animals.
Innovation Solution
Isolation and selection of lactic acid bacteria strains from the feline gastrointestinal tract, such as Lactobacillus salivarius, Lactobacillus animalis, and Lactobacillus reuteri, with specific 16s-23s polynucleotide sequences, for use in probiotic compositions that promote gastrointestinal health and immune system modulation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If probiotic bacteria are derived from human sources, then they may be effective for human health, but they are not specifically adapted for feline gastrointestinal health
Solution Approach 1:
The patent applies local quality by isolating probiotic bacteria specifically from the feline upper gastrointestinal tract rather than using generic human-derived strains. This ensures the bacteria are locally adapted to the feline GI environment, matching the specific physiological conditions of cats while maintaining probiotic effectiveness.
Solution Approach 2:
The patent employs parameter changes by selecting bacteria based on their specific origin location within the feline GI tract (upper portion including stomach and small intestine) and their ability to survive processing conditions. This targeted selection optimizes the bacterial strains for feline-specific parameters such as gastric acidity tolerance and bile resistance.
2Reliability
If bacteria are isolated from faecal samples, then they may show probiotic activity, but they do not represent the natural microflora of the upper GI tract
Solution Approach 1:
The patent applies preliminary action by isolating bacteria directly from the upper GI tract before they reach the faeces, capturing the natural microflora composition of the target location. This preliminary isolation ensures the bacteria represent the actual inhabitants of the feline upper GI tract rather than contaminants or lower tract organisms.
Solution Approach 2:
The patent uses extraction by specifically removing and isolating bacteria from the upper portion of the feline GI tract (stomach and small intestine) separate from faecal samples. This extraction method captures the genuine upper GI microflora population that would naturally be present in healthy cats.
3Reliability
If probiotic strains are selected for survival during processing, then they maintain viability, but selection criteria become more complex
Solution Approach 1:
The patent applies partial action by implementing a multi-stage selection process that tests for specific survival capabilities (gastric acidity tolerance, bile resistance, heat stability) rather than requiring all possible stress resistance. This partial selection approach ensures adequate processing survival without unnecessarily complicating the selection criteria.
Data Source
AI summary
According to the invention there is provided a strain of lactic acid bacteria of the genus Lactobacilli obtainable by isolation from resected and washed feline gastrointestinal tract having a probiotic activity in animals. Methods of use and compositions comprising the Lactobacilli of the present invention are also provided.