Bacteria TRIZ Cases

Whole-cell biosynthesis of 2′-fucosyllactose reduces purification costs by integrating GDP-L-fucose synthesis with alpha-1,2 fucosyltransferase activity.

Isolating lactic acid bacteria from the feline upper gastrointestinal tract ensures natural microflora representation and reliable probiotic activity.

Upregulating specific genetic regions like yfdE in E. coli resolves the contradiction between high ethanol productivity and alcohol toxicity.

Lactobacillus plantarum 121-5 replaces chemical catalysis with mild bioconversion, eliminating equipment corrosion and environmental pollution.

Engineered host cells eliminate periplasmic proteases to prevent SpyTag cleavage, ensuring reliable covalent ligation with SpyCatcher.

Bacillus coagulans strains convert xylitol byproduct sugars into L-lactic acid, resolving the contradiction between high yield and production cost.

Lactococcus bacterium composition alleviates physiological fatigue and reduces rest desire through daily intake.

Expressing RpaB or SrrA decoy proteins in cyanobacteria removes regulatory bottlenecks, increasing carbon fixation and biomass production.

DtpA protein prevents protein misfolding and aggregation during desiccation, maintaining structural integrity without inducing chemical degradation.

Surface layer protein A from Lactobacillus acidophilus binds dendritic cell receptors to induce regulatory immune responses.

Adding methanotrophic bacteria to fermented feed colonizes the ruminant digestive tract, consuming methane before it enters the atmosphere.

Deleting reductase genes and adding DsbC catalysts creates an oxidizing cytoplasm that stabilizes disulfide bonds, preventing protein degradation.

Segmenting the phage genome from the host strain eliminates safety risks from pathogenic bacteria while maintaining high productivity.

Chromosomal gene integration stabilizes recombinant microbial strains, eliminating antibiotic dependence and boosting productivity to 110.4 g/L.

Cys to Ser mutations in recombinant polypeptides increase cellulase activity and thermostability for biomass conversion.

Anaerobic fermentation of carbon monoxide using Clostridium autoethanogenum produces 2,3-butanediol at high rates.

Engineered microorganisms boost glycine availability to enhance guanidinoacetic acid production titers.

Engineered VNP20009-M bacteria deliver methioninase to deplete methionine in tumors, solving the trade-off between antitumor efficacy and systemic toxicity.

Lactococcus lactis LL358 and Lactobacillus salivarius LS159 composition modulates gut microbiota to support renal health.

Segmented polypeptide antigens replace whole-cell preparations to eliminate cross-reactivity and improve diagnosis accuracy.

Multizyme constructs and down-regulated peroxisome biogenesis in Yarrowia lipolytica increase EPA concentration while suppressing linoleic acid byproducts.

A Brevibacillus agri strain produces lipopeptide biosurfactants that reduce crude oil surface tension and viscosity.

Alditol oxidase converts D-glucose and D-glyceraldehyde to pyruvic acid using only three enzymes without coenzymes.

A pharmaceutical formulation containing tomatidine, rapamycin, and xylitol modifies the gut microbiome to enhance bone strength.

Recombinant E. coli strains express Antarctic bacterial photolyases to provide high-purity DNA repair enzymes without cyanobacterial lipopolysaccharides.

Isolated Lactobacillus brevis CNCM I-5321 strain inhibits tumor cell proliferation and modulates proapoptotic gene expression.

Engineered Corynebacterium glutamicum with optimized medium resolves isomer purity and scalability trade-offs for cost-effective astaxanthin production.

Alkaline oxidizer treatment degrades nucleic acids in PHA culture solutions to lower viscosity for easier polymer collection.

Morganella bacterial vesicles serve as biomarkers for disease detection and therapeutic agents to suppress inflammatory responses.

Recombinant E. coli overexpresses phosphoglucosamine mutase and aminotransferase to increase fructosylated chondroitin yield by 108.9%.

A toxin-antidote genetic circuit represses bacterial survival in high-glucose normal tissues, resolving systemic toxicity and improving tumor specificity.

Akkermansia muciniphila SSYD-3 resolves fermentation stability trade-offs by improving acid resistance and storage durability.

Heat-resistant Bacillus coagulans spores survive baking to colonize the gut, solving instability issues.

A plasmid marker composition utilizes essential metabolic pathway genes to select living modified organisms without antibiotic resistance markers.

nZVI boosts Pseudomonas JD37 ROS production to degrade PCBs, shortening remediation time without secondary pollution.

Optimized signal peptide coding sequences encode mRNA with hairpin structures to enhance protein expression and secretion.

Synthetic culture medium replaces animal-derived components with soy protein hydrolysate to enable large-scale production for pharmaceutical applications.

Culturing rpoS-inactivated Pantoea at pH 3-5 suppresses competing stress pathways, resolving the trade-off between productivity and survival.

Combining cefotetan and ceftriaxone stabilizes the medium against room temperature degradation while maintaining high sensitivity for accurate MRSA screening.

Deleting spinactin biosynthesis genes in Saccharopolyspora spinosa reduces impurities and lowers purification costs while maintaining high spinosyn yields.

Directed evolution modifies probiotic lipid metabolism to inhibit absorption and manage body weight.

Deleting ldhA and pflB genes reduces by-product formation to improve 2,3-butanediol yield.

Isolating Bifidobacterium from the feline stomach and small intestine resolves the trade-off between probiotic activity and natural microflora presence.

L-isoleucine and L-valine activate super-dormant Bacillus spores, achieving 50% germination within one hour.

Engineered strains bypass the malonyl-ACP step to increase fatty acid production rates and optimize carbon flow.

Replacing chemical bonding with electrostatic adsorption preserves protein spatial structure while enabling efficient bacterial capture.

Membrane scaffold proteins enable proper folding of insoluble CYP11A1 in Bacillus megaterium, resolving prokaryotic expression bottlenecks.

Recombinant yeast expresses specific bacterial xylose isomerases to resolve insufficient enzyme activity and enable efficient xylose utilization.

Pre-coating Bacillus spores with amino acids overcomes low pH inhibition to ensure rapid gastrointestinal germination.