Fish Scale Decomposition Using Bacillus Velezensis Fermentation
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Solution Overview
Problem
Existing methods for decomposing fish scales are limited by the availability of microorganisms capable of efficient decomposition, primarily focusing on collagen acquisition, and lack alternatives for large-scale production of peptides and amino acids.
Innovation Solution
A mass production method using Bacillus velezensis (DSM 34894) is employed, involving culturing the bacteria with fish scales in a specific medium at 37°C for 12-24 hours, resulting in a high yield of peptides and amino acids.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If traditional enzymatic hydrolysis followed by acid treatment is used to decompose fish scales, then collagen can be obtained, but the process is complex and requires multiple steps including high-energy acid treatment
Solution Approach 1:
The patent extracts and utilizes the collagenase enzyme directly from Bacillus velezensis bacteria to decompose fish scales, eliminating the need for complex multi-step processes including acid treatment. This single-enzyme approach simplifies the overall process while maintaining high collagen yield
Solution Approach 2:
The patent introduces Bacillus velezensis bacteria as an intermediary organism that produces collagenase enzyme to facilitate fish scale decomposition. This biological intermediary replaces the need for direct acid treatment and multi-step enzymatic processes, simplifying the overall methodology
2Quantity of substance
If collagen is obtained through traditional decomposition methods, then high molecular weight collagen is produced, but it cannot be used directly by organisms and requires further decomposition into polypeptides
Solution Approach 1:
The patent employs a continuous decomposition process where Bacillus velezensis bacteria progressively break down fish scales into peptides and amino acids. This continuous action ensures that the final product is in a form directly usable by organisms, eliminating the need for separate decomposition steps
Solution Approach 2:
The patent controls the decomposition parameters including temperature (37°C), time (12-24 hours), and bacterial concentration to optimize the conversion of collagen into usable peptides and amino acids. These parameter adjustments ensure the product is directly applicable to biological systems
3Device complexity
If only limited microorganisms are used for fish scale decomposition, then the process is straightforward, but there are few alternatives and limited productivity
Solution Approach 1:
The patent optimizes critical parameters including temperature (37°C), time (12-24 hours), and bacterial concentration (7-10% w/v) to maximize decomposition efficiency. These parameter adjustments enable Bacillus velezensis to achieve high productivity while maintaining process simplicity
Solution Approach 2:
The patent demonstrates that Bacillus velezensis can decompose fish scales into multiple useful products including peptides, amino acids, and collagen, making it a multi-functional microorganism that replaces the need for multiple specialized organisms or process steps
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Bacillus velezensis effectively decomposes fish scales in large-scale fermentation, producing high concentrations of peptides and amino acids suitable for industrial applications in agriculture, skincare, health supplements, and medical materials.
Implementation Method 1
A presently available method for decomposing fish scales involves enzymatic hydrolysis subsequent to acid treatment to yield collagen
Implementation Method 2
The Bacillus velezensis newly isolated by the method of the present invention possesses significant capability for decomposing fish scales, enabling its application in large-scale fermentation
Data Source
AI summary
A mass production method using Bacillus velezensis for decomposition of fish scales and product of the same is provided. The Bacillus velezensis is deposited in Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH with an accession number of DSM 34894. The mass production method includes the steps of culturing a bacterial solution of Bacillus velezensis, mixing the bacterial solution with a fish scale material to get a mixed solution, and allowing the mixed solution to react at 37° C. for 12-24 hours for decomposition of the fish scale material. The present mass production method not only can be used to decompose a larger quantity of fish scales but also applied to mass production of a fish scale decomposition product. Thereby the present method has industrial application value.