Flavanone 2-Hydroxylase Gene for Blue Flower Pigment Pathways
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Solution Overview
Problem
Existing technologies have not effectively utilized enzymes belonging to the 2-oxoglutaric acid-dependent dioxygenase (ODD) family for hydroxylating the 2-position of flavanones, limiting the development of transgenic plants with improved stress resistance and modified flower colors, particularly blue flower colors.
Innovation Solution
Identification and utilization of polynucleotides encoding enzymes from the ODD family, specifically from Caryophyllaceae plants, which exhibit hydroxylating activity at the 2-position of flavanones, enabling the production of 2-hydroxyflavanone and enhancing flavone C-glycoside accumulation in plants.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If enzymes from P450 family or general ODD family are used for flavanone hydroxylation, then flavone biosynthesis can occur, but specific hydroxylating activity at the 2-position of flavanones cannot be achieved
Solution Approach 1:
The patent identifies and utilizes a specific enzyme within the ODD family that possesses localized specialized function for hydroxylating the 2-position of flavanones. This enzyme exhibits distinct catalytic properties compared to other ODD family members, demonstrating that specific local characteristics within a protein family can confer unique functional capabilities necessary for producing blue flower colors.
Solution Approach 2:
The patent changes the key parameter of enzyme selection from general ODD family or P450 family enzymes to a specific ODD family enzyme with confirmed 2-position hydroxylating activity. This parameter change in enzyme specificity enables the desired biochemical reaction pathway for flavone C-glycoside accumulation and blue color production.
2Shape
If transgenic plants are created to produce blue flower colors, then flower color modification is achieved, but stress resistance improvement may be compromised
Solution Approach 1:
The patent utilizes the plant's own endogenous ODD family enzyme pathway to produce blue pigments through flavone C-glycoside accumulation. By leveraging the plant's native metabolic capabilities rather than introducing foreign enzymes, the transgenic approach maintains compatibility with the plant's physiological systems and preserves stress resistance while achieving the desired phenotypic modification.
3Productivity
If non-human hosts are used for enzyme production, then production efficiency is improved, but enzyme activity may be reduced
Solution Approach 1:
The patent optimizes expression parameters in non-human hosts to maintain enzyme activity. By carefully controlling expression conditions and utilizing the specific properties of the identified ODD family enzyme, the system achieves high productivity in heterologous hosts while preserving the critical 2-position hydroxylating activity necessary for functional flavone biosynthesis.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution allows for the creation of transgenic plants with improved stress resistance and modified blue flower colors, as well as efficient production of 2-hydroxyflavanone using non-human hosts like yeast or bacteria.
Implementation Method 1
enzymes belonging to the ODD family exhibit activity of specifically hydroxylating the 2-position of flavanones
Data Source
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AI summary
Provided is a transgenic plant that has improved stress resistance and/or modified flower color, and modification to a blue flower color, or its inbred or outbred progeny, or their propagules, partial plant bodies, tissue or cells, as well as a method which allows easy and efficient production of 2-hydroxyflavanone. There is used a polynucleotide encoding an enzyme that belongs to the 2-oxoglutaric acid-dependent dioxygenase (ODD) family and that has hydroxylating activity at the 2-position of flavanones.