Immobilizing Fluidic Sample to Prevent Spatial Broadening
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Solution Overview
Problem
The phenomenon of spatial broadening of fluidic samples before and during separation in sample separation devices, such as liquid chromatography and gel electrophoresis, leads to reduced spatial accuracy and separation efficiency.
Innovation Solution
A method and device that temporarily immobilize fluidic samples using an immobilizing agent, forming a bead or gel matrix to prevent spatial broadening, followed by controlled release using electric or thermal means to restore mobility for accurate separation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Loss of time
If the fluidic sample is kept in a waiting state before separation, then the separation process can be properly prepared and initiated, but spatial broadening occurs which deteriorates spatial accuracy of sample separation
Solution Approach 1:
The patent applies preliminary action by forming a gel matrix structure before the separation process to prevent spatial broadening during the waiting period. The gel matrix is prepared in advance and the sample is introduced into this pre-formed structure, allowing the system to be ready for separation without the sample diffusing or broadening spatially during the waiting time.
Solution Approach 2:
The patent utilizes parameter changes by transitioning the medium from a liquid state to a gel state during the waiting period, then reversing this transition during separation. The gel formation is triggered by changing physical or chemical parameters (such as temperature, pH, or adding crosslinking agents), which immobilizes the sample and prevents spatial broadening. During separation, these parameters are changed again to restore sample mobility.
2Manufacturing precision
If an immobilizing agent is used to prevent spatial broadening, then spatial accuracy is improved, but additional steps for immobilization and release are required
Solution Approach 1:
The patent applies phase transitions by utilizing the gel-to-liquid transition of the immobilizing agent to control sample immobilization and release. The immobilizing agent transitions from a liquid state to a gel state to immobilize the sample, preventing spatial broadening. Subsequently, a reverse phase transition from gel to liquid state releases the sample for separation. This phase transition mechanism provides a simple, integrated system that combines immobilization, sample holding, and release functions without requiring complex mechanical or chemical intervention systems.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach effectively prevents spatial broadening during waiting times, maintaining sample integrity and enhancing separation accuracy by ensuring precise injection and analysis of fluidic samples in sample separation devices.
Implementation Method 1
immobilizing the fluidic sample by an immobilizing agent (in particular by forming at least one bead comprising fluidic sample and immobilized immobilizing agent)
Implementation Method 2
releasing or removing the fluidic sample from the (in particular bead comprising the) immobilizing agent (in particular to restore or re-establish free movability of the fluidic sample)
Data Source
AI summary
A method of handling a fluidic sample in a sample separation device includes at least partly immobilizing the fluidic sample by an immobilizing agent inhibiting spatial broadening of the fluidic sample, and subsequently at least partly releasing the fluidic sample from the immobilizing agent.