Fluorescence Spectrum Separation for Multi-Dye Particle Analysis
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Conventional techniques struggle to accurately separate measurement spectra of particles labeled with multiple fluorescent dyes due to overlapping fluorescence spectra, leading to errors in fluorescence intensity measurements.
Innovation Solution
A particle analysis system utilizing a weighted least squares method (WLSM) to calculate fluorescence intensity by performing separation processing on measurement spectra using a plurality of photodetectors, with weights determined based on variations in measured values.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If multiple fluorescent dyes are used to label microparticles for detailed analysis, then the analysis capability is improved, but fluorescence spectrum overlap occurs leading to measurement errors
Solution Approach 1:
The patent segments the overlapping fluorescence spectrum into individual dye contributions by measuring the spectrum at multiple wavelength bands and mathematically separating the signals. Each photodetector measures a specific wavelength band, and the information processing unit separates the composite spectrum into individual fluorescent dye components, enabling precise measurement of each dye's fluorescence intensity despite spectral overlap.
2Measurement precision
If conventional mathematical separation methods are used to correct fluorescence intensity errors, then some error correction is achieved, but high-accuracy separation of measurement spectrum remains difficult
Solution Approach 1:
The patent adds the dimension of wavelength by measuring fluorescence intensity across multiple wavelength bands instead of using a single broad-band measurement. This spectral dimension allows the information processing unit to distinguish between overlapping fluorescent dyes by analyzing their unique spectral signatures at different wavelengths, achieving high-accuracy separation that conventional single-point measurement methods cannot provide.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables high-accuracy separation of fluorescence intensities from each fluorescent dye, reducing errors and improving measurement precision.
Implementation Method 1
a plurality of photodetectors configured to acquire light generated by irradiating a particle labeled with a plurality of fluorescent dyes with excitation light
Data Source
AI summary
A measurement spectrum obtained by irradiating a particle labeled with a plurality of fluorescent dyes with excitation light is separated for each fluorescent dye with high accuracy. Provided is a particle analysis system (1) including a plurality of photodetectors (23) configured to acquire light generated by irradiating a particle labeled with a plurality of fluorescent dyes with excitation light; and an information processing unit (132) configured to calculate fluorescence intensity of each fluorescent dye by performing separation processing on a measurement spectrum based on measured values from the plurality of photodetectors (23) with a single staining spectrum of each fluorescent dye, in which the separation processing is performed by using a weighted least squares method (WLSM) including a weight determined based on a variation in the measured values.